商陆组织培养及其快速繁殖研究  

Study on the Tissue Cultivation and Rapid Propagation of the Phytolacca acinosa Roxb

作　　者：张丽珍[1] 杨冬业[2] 谭梅珍[1] 刘杰[3] 

机构地区：[1]桂林师范高等专科学校化学与工程技术系,广西桂林541001 [2]桂林医学院,广西桂林541004 [3]桂林理工大学,广西桂林541004

出　　处：《种子》2014年第2期1-5,共5页Seed

基　　金：国家自然科学基金资助项目(编号:41001186)

摘　　要：以商陆茎段为外植体,研究其离体培养再生途径。结果表明,MS+6-BA 1.0 mg/L+IBA 0.05 mg/L适合丛生芽诱导,诱导率达100%;MS+6-BA 1.0 mg/L+IBA 0.02 mg/L适合继代增值培养;1/2 MS+IBA 0.5 mg/L适合生根诱导,诱导率为86.7%;组培苗移栽成活率达100%。The stems with bugs of Phytolacca acinosa Roxb as explants were cultured on MS basal medium. The results showed that MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.05 mg/L are suitable for inducing adventitious bugs, the induced rate reaches 100%. The perfect medium for adventitious shoot proliferation is the MS medium MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.02 mg/L. 1/2 MS ＋ IBA 0.5 mg/L is the best suitable medium for inducing roots and the induced rate reaches 86.7%. When the regenerated plantlets transferred to an open field grow, the survival rate reaches 100% and showed excellented growth.

关 键 词：商陆 组织培养 愈伤组织 快速繁殖 

分 类 号：S567.23[农业科学—中草药栽培]