不同浓度NaCl胁迫后2种盐生植物叶片类囊体膜H^+-ATPase活性分析  

Effect of NaCl Stress on H^+-ATPase Activity in Leaves from Two Types of Halophytes

作　　者：贾晋[1] 代莉慧[1] 倪彬[1] 魏光普[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头014010

出　　处：《种子》2014年第2期35-38,共4页Seed

基　　金：内蒙古科技大学产学研合作项目"耐低温;高光效番茄育种材料筛选及新品种选育"(编号:PY-201116);包头市科技普及项目"包头市番茄黄化曲叶病毒病防治知识的普及"(编号:2012 S 2008-6)

摘　　要：以不同浓度NaCl胁迫后盐爪爪和碱蓬叶片为材料,研究了2种盐生植物类囊体膜H+-ATPase活性变化趋势。结果表明,随NaCl浓度升高,2种盐生植物类囊体膜H+-ATPase活性均呈先升后降的变化趋势。NaCl浓度为100 mmol/L时,碱蓬和盐爪爪叶片类囊体膜H+-ATPase活性分别比未胁迫升高了38%和102%。NaCl浓度大于300 mmol/L时碱蓬叶片类囊体膜H+-ATPase活性显著低于对照;而盐爪爪叶片类囊体膜H+-ATPase活性在NaCl达到500 mmol/L时低于对照但无显著性差异。此外,盐爪爪叶片类囊体膜H+-ATPase活性显著高于碱蓬,表明盐爪爪的耐盐能力显著高于碱蓬。Using Kalidium foliatum leaves stressed by different NaCl concentrations as material, H ~ -ATPase activity in the thylakoid membrane from two types of halophytes was studied. The results showed that with the increasing of NaCl concentration, H ＋ -ATPase activity of the halophytes leaves first increased then decreased, the H ＋ -ATPase activities in Suaeda glauca and Kalidium foliatum.leaves significantly increased about 38% and 102% by 100 mmol/L NaCl stress. The H ＋ -ATPase activity in Suaeda glauca were significantly lower than the untreated plant when NaCl concentration was higher than 300 retool/L, but H ＋ -ATPase activity in K. foliatum was no significantly lower than the untreated plant under 500 mmol/L NaCl. H ＋ -ATPase activity in the thylakoid membrane of K. foliatum leaves was significantly higher than S. glauca, which meant the salt tolerance in K. foliatum was significantly higher than S. glauca.

关 键 词：NACL胁迫 盐爪爪 碱蓬 类囊体膜 H+-ATPase 

分 类 号：Q945.78[生物学—植物学]