基于X射线相位衬度成像技术的兔眼球血管重建  

Three-dimensional reconstruction of rabbit eye vessels based on X-ray phase contrast imaging technique

作　　者：张璐[1] 崔倩倩[1] 张昆亚[1] 赵秋云[1] 钱秀清[1] 全海英[1] 刘志成[1] 

机构地区：[1]首都医科大学生物医学工程学院,北京100069

出　　处：《医用生物力学》2014年第1期46-52,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目(11102123;31070840);北京市教育委员会科技计划面上项目(KM 2011100 25009);北京市属市管高校人才强教计划资助项目(PHR201110506);北京市自然科学基金项目(3122010)

摘　　要：目的利用X射线相位衬度成像技术构建兔眼血管网的三维可视化模型,观测兔眼虹膜血管的形态学特征。结果用硫酸钡对新西兰白兔眼球血管进行造影,利用X射线相位衬度成像技术采集离体兔眼样本的高精度投影图像。图像经由滤波反投影法重建出断层图像,利用Amira 5.2.2软件进行三维重建。方法高精度投影图像中眼球主干血管清晰连贯,能够观测到部分细小血管的分布及走向,可分辨的最小血管直径约为10μm;CT扫描图像三维重建后得到兔眼血管网的三维模型,精确到虹膜动脉大环4级分支结构,最小血管直径可达40μm。结论利用X射线相位衬度成像技术可以比较清楚地观察到兔眼血管,并且能够在一定程度上构建出血管网的三维可视化模型,为眼球内血管血流动力学分析提供依据,对青光眼的临床研究具有参考价值。Objective To establish a three-dimensional （3D） visualization model for the vessel system of rabbit eyes using X-ray phase contrast imaging（XPCI）technique, and observe the morphological characteristics of iris vessels of the rabbit eyes. Methods Angiography on vessels of the New Zealand rabbit eyes was conducted using Barium sulfate as the contrast medium. The projected images of in vitro rabbit eye samples with high precision were obtained by XPCI technique, and then converted to tomography images by filter back projection. The 3D reconstruction of the rabbit eyes was completed by commercial visualization software Amira 5.2.2. Results The main blood vessels of the rabbit eyes were clear and coherent in the projection images, and the distribution and trend of some small vessels could be observed, with the smallest distinguishable blood vessel diameter being about 10 μm. The 3D model for vessel network of the rabbit eyes was built after 3D reconstruction of CT scan images. The major arterial circle of the iris could be observed at level 4 branch structure of vessels in the fundus, and the minimum diameter of vessels that could be identified was 40 μm. Conclusions The vessels of the rabbit eyes can be clearly observed and 3D visualization of vessel network can be constructed by using XPCI technique, which would provide basis for the analysis on hemodynamics of blood vessels in the eye and reference for the clinical study of glaucoma.

关 键 词：相位衬度成像 血管重建 三维可视化 血流动力学 

分 类 号：R318.01[医药卫生—生物医学工程]