大鼠两种脊髓半切模型制备方法的改良及比较  

Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats

作　　者：赵富生[1] 张晓莉[1] 武庚[1] 陈斌[1] 李月珍[1] 金秀东[1] 张际绯[1] 

机构地区：[1]牡丹江医学院,牡丹江157011

出　　处：《实验动物与比较医学》2014年第1期6-11,共6页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（81171467）

摘　　要：目的 比较两种大鼠脊髓半切模型制作方法的各自特点及其应用,为临床脊髓损伤研究提供适宜的动物模型.方法 60只SD大鼠随机分为脊髓半横切组（A组,n=20）、脊髓楔型块状半切组（B组,n=20）和正常对照组（C组,n=20）.术后采用BBB动功能评分、斜板实验、HE染色和MRI检测综合评价两种脊髓半切模型的稳定性.结果 术后A组和B组大鼠伤侧后肢BBB运动功能评分及斜板维持最大角度值显著低于C组（P＜0.05）,而这些指标在A组和B组之间没有明显差异;术后28dHE和MRI检测示A组和B组间脊髓损伤区组织结构具有显著差异.结论 脊髓半横切与脊髓楔型半切法均可建立稳定、可靠的脊髓半切伤模型,而脊髓楔型半切模型更适于评价移植物治疗脊髓损伤的研究.Objective Comparing the characteristics and application of two methods for establishing the hemi-sected spinal cord injury model of rats and provide suitable spinal cord injury models for clinical research. Methods Sixty Sprague-Dawley rats were randomly divided into transverse-hemisected group （A group, n=20）, wedge-shaped-block-hemisected group （B group, n=20） and normal control group （C group, n=20）. After operation, the stability of the two hemi-sected models were comprehensive assessed by Basso-Beattie-Bresnahan （BBB） scores, inclined plane method, HE staining and MRI detection. Results After operation, the BBB scores and maximum angle of inclined Plane method of rats in group A and group B were significantly lower than group C （P〈0.05）, but these indexes had no significant difference between group A and group B; In addition, HE staining and MRI detection show a significant morphological change between group A and group B 28 days after operation. Conclusion Both meth- ods of transverse-hemise~ ted and wedge-shaped block-hemisected can establish a stable and reli- able spinal cord injury model, and wedge-shaped-block-hemisected model is more suitable to assess graft treatment of spinal cord injury.

关 键 词：脊髓半切 动物模型 大鼠 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]