三个封闭群实验兔线粒体DNA D-loop区多态性分析  

Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits

作　　者：申幸娇[1] 岳秉飞[1] 马丽颖[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《实验动物与比较医学》2014年第1期29-34,共6页Laboratory Animal and Comparative Medicine

基　　金：“国家科技支撑计划”（2011BAI15801：2013BAK11801）

摘　　要：目的 通过对三个封闭群实验兔线粒体DNA控制区序列进行分析,从分子水平探讨其群体的多样性和均一性.方法 利用特异引物对新西兰白兔、日本大耳白兔、青紫蓝兔三个封闭群共71份血液样品线粒体D-loop区部分序列进行扩增,纯化,测序,用Mega4.0和DNA SP软件对变异位点数、简约信息位点数、单倍型、单倍型多样度、核苷酸多样度等遗传信息进行分析.结果 在71份样品中,共检测出了6个多态性位点,多为C→G突变,定义了9种单倍型,且三个种群分别有各自单倍型,互不重叠.三个种群D-loop区多态性及种群多样性均偏低.结论 本研究为兔线粒体D-Loop区序列特征和国内常用三个封闭群实验兔种群结构提供了更多数据,并为遗传育种提供指导.Objective To discuss the genetic diversity and uniformity in three groups of closed colony rabbits based on mitochondrial DNA control region. Methods Seventy-one blood samples from New Zealand rabbit, Japanese White rabbit and Chinchilla rabbit were collected for amplifying ,purifying and sequencing the part of sequences of mitochondrial DNA control region by PCR and Genetic Analyzer. The variation sites, Parsimony informative sites, haplotypes, haplotype diversity, nucleotide diversity were analyzed by Mega 4.0 and DNA SP. Results 6 diversity sites and 9 haplotypes were found in the seventy-one rabbits, and most mutations were C ~ G. Every population had its own haplotypes. The polymorphism of Mitochondrial DNA Control Region and population diversity in three group were both low. Conclusions More sequence information about mtDNA sequences and population structure of laboratory rabbits were supplied,which was helpful to genetic breeding.

关 键 词：封闭群兔 单倍型 多态性 

分 类 号：Q95-33[生物学—动物学]