IFN-γ对HAART治疗1年的HIV／AIDS患者Treg细胞的调节作用  

Regulatory effects of IFN-γ on Treg cells from HIV/AIDS patients receiving HAART for one year

作　　者：张权[1] 姚运海[1] 何艳[1] 周泉[1] 郑煜煌[1] 曾飔[1] 

机构地区：[1]中南大学湘雅二医院感染科,长沙410011

出　　处：《中华微生物学和免疫学杂志》2014年第1期15-18,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家“十二五”重大科技专项（分课题）（2012ZX10001-003）

摘　　要：目的探索IFN-γ对高效抗逆转录病毒治疗（HAART）1年的HIV／AIDS患者Treg细胞的调节作用。方法对30例CD4^＋T细胞数〈350个／μl的HIV／AIDS患者进行HAART治疗，并分别于HAART治疗前（0周）、治疗24周及48周备血，分离外周血单个核细胞（PBMC），随机分成2组进行无菌培养，一组直接培养，另一组加IFN-γ（40pg／ml）培养，培养5d后分别收集上清液和细胞。ELISA检测上清液IL-12，流式细胞术检测CD4^＋CD25^＋Foxp3 Treg细胞。结果HAART治疗前、治疗24周、48周PBMC培养上清液中IL-γ2水平（单纯培养VS共培养组）分别为：[（37．02±12．76）VS（41．79＋15．02），t=2．336，P=0．03]、[（41．76±17．01）vs（47．2±14．26），t=2．702，P=0．014]、[（48．01±11．84）vs（53．44±11．30），t=3．14，P=0．003]；培养细胞中CD4^＋CD25^＋Foxp3 Treg细胞占CD4^＋T细胞比例（单纯培养vs共培养组）分别为：[（10．41±1．10）vs（2．40±1．11）％，t=13．89，P=0．000]、[（8．33±2．03）VS（1．99±0．86）％，t=12．93，P=0．000]、[（5．65±1．55）VS（1．32±0．73）％，t=10．61，P=0．000]。且同组HAART治疗不同时间点间比较差异均有统计学意义（P〈0．05）。结论随着HAART治疗时间的延长，HIV／AIDS患者IL．12水平逐渐升高，Treg细胞比例逐渐下降，IFN-γ起重要的免疫调节作用。Objective To investigate the regulatory effects of IFN-γ on Treg cells from HIV/AIDS patients receiving highly active antiretroviral therapy （HAART） for one year. Methods Thirty HIV/AIDS patients whose CD4^＋T cells were below 350/μl were recruited for HAART therapy. Blood samples were col- lected at the time points of 0, 24, 48 weeks after HAART. PBMCs were isolated and randomly divided into two culture groups. One group was cultured directly in medium and another group was co-cultured with IFN-γ （40 pg/ml）. The supernatants and cells were separated after 5 days of culture for analysis. The con- centrations of IL-12 and CD4^＋CD25^＋Foxp3 Treg cells were measured by ELISA and flow eytometry, respec- tively. Results The levels of IL-12 in the supernatants from the culture without IFN-γat time points of 0, 24, 48 weeks after HAART were lower than those from the co-cultured group [ （37.02±12.76） vs （41.79±15.02）, t=2.336, P=0.03; （41.76±17.01） vs （47.2±14.26）, t=2.702, P=0.014; （48.01＋ 11.84） vs （53.44±11.30）, t=3. 14, P=0. 003]. The percentages of CD4^＋CD25^＋Foxp3 Treg cells in CD4^＋ T cells from the direct-cultured group were higher than those from the co-cultured group at the three time points [（10.41±1.10）% vs （2.40＋1.11）%, t=13.89, P=0.000; （8.33±2.03）% vs （1.99± 0.86）%, t=12.93, P=0.000; （5.65±1.55）% vs （1.32±0.73）%, t=10.61,P=0.000]. Moreover, the results within the same group at the time points of 0, 24, 48 weeks upon HAART were also significantly different. Conclusion With the interference of HAART, IL-12 levels were increased, while CD^＋CD25^＋ Foxp3 Treg cells were decreased in patients with HIV/AIDS. IFN-γ plays an important role in this process.

关 键 词：IFN-Γ HIV AIDS 高效抗反转录病毒治疗 CD4^+CD25^+Foxp3调节性T细胞 白 介素12 

分 类 号：R512.91[医药卫生—内科学]