四种常用细菌感染指标的动物实验研究  

Study on four circulating inflammatory parameters in animal model of systemic infection

作　　者：谢尹晶[1,3] 段晋燕[1] 张洪瑞[3] 刘一凡[1] 唐红卫[1] 吴小利[3] 罗燕萍[2] 杨继勇[2] 王成彬[1] 

机构地区：[1]解放军总医院临床检验科,北京100853 [2]解放军总医院微生物科,北京100853 [3]温州医科大学检验医学院生命科学学院临床检验诊断学,325035

出　　处：《中华微生物学和免疫学杂志》2014年第1期29-33,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的监测全身感染性疾病小鼠模型血液中的相关炎症指标，探讨它们在鉴别诊断革兰阴性菌和革兰阳性菌感染方面的价值。方法分别通过尾静脉注射大肠埃希菌和金黄色葡萄球菌建立小鼠的革兰阴性菌和革兰阳性菌全身感染模型，在感染后1h、3h、6h、10h、12h、1d、2d、5d处死各组小鼠，检测其血液白细胞计数（white blood cell count，WBC）、c-反应蛋白（C-reactive protein，CRP）、降钙素原（procalcitonin，PCT）和白细胞介素6（interleukin-6，IL-6）。结果大肠埃希菌感染组和金黄色葡萄球菌感染组小鼠，其WBC均在感染后10h明显升高，分别为（1．9±0．52）×10^9／L，（2．1±0．70）×10^9／L；CRP均在感染后3h明显升高，分别为（258．1±13．4）ng／ml，（233．5±59．9）ng／ml；PCT也在感染后3h明显升高，分别为（1824．6±331．4）pg／ml，（594．4±93．0）pg／ml；IL-6在大肠埃希菌感染后1h明显升高，为（226±34．2）pg／ml，阴性对照组和金黄色葡萄球菌感染组的IL-6水平均低于检测下限；4种指标的ROC曲线分析显示，两组IL-6的ROC曲线下面积（areas under the curve，AUC）差异有统计学意义，其他指标无差异。结论IL-6、CRP和PCT在感染早期即明显升高，且IL-6水平在大肠埃希菌和金黄色葡萄球菌引发的感染中有显著差异。Objective To explore the possibility of using four circulating inflammatory parameters for the differential diagnosis of systemic gram-negative and gram-positive bacterial infections in mouse models. Methods Escherichia coli and Staphylococcus aureus were respectively inoculated into mice through tail vein to establish the animal models of systemic infection by representative gram-negative and gram-positive bacteria. Four parameters including the levels of white blood cell counts （WBC）, C-reactive protein （CRP）, procalcito- nin （PCT） and interleukin 6 （IL-6） in blood samples were detected at 1,3, 6, 10, 12 hours, as well as 2 and 5 days after bacteria inoculation. Results Levels of WBC in mice inoculated with Escherichia coli and Staphylo- coccus aureus were increased significantly at 10 hours after inoculation, which were （ 1.9 ± 0.52） ×10^9/L and （2.1±0.70）×10^9/L respectively. Levels of CRP [ （258.1±13.4） ng/ml and （233.5±59.9） ng/ml] and PCT [ （1824.6±331.4） pg/ml and （594.4±93） pg/ml] were all significantly up-regulated at 3 hours after in- oculation. Levels of IL-6 were transiently increased in mice inoculated with Escherichia coli at the time point of 1 hours [ （226±34.2） pg/ml], but were below detectable limit at any other time points as compared with those in control group and Staphylococcus aureus infected group. The ROC curves of four parameters indicated that only the areas under the curve （AUC） of IL-6 showed significant differences between two infections. Conclusion Levels of IL-6, CRP and PCT were significantly increased in early stage of bacterial infection. IL-6 level showed significant difference between Escherichia coli and Staphylococcus aureus infections.

关 键 词：感染 白细胞计数 C-反应蛋白 降钙素原 白细胞介素6 

分 类 号：R446.5[医药卫生—诊断学]