HIV-1膜蛋白gp120V3环的神经毒性研究  

Neurotoxicity of HIV-1 envelope protein gp120 V3 loop

作　　者：张志丽[1] 陈兵刚[2] 姜太一[1] 乔录新 吴昊[1] 陈德喜 张玉林[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心,北京100069 [2]山东省滨州市中心医院感染内科,山东滨州251700 [3]北京市肝病研究所,北京100069

出　　处：《首都医科大学学报》2014年第1期87-91,共5页Journal of Capital Medical University

基　　金：国家自然科学基金(81371399;81272266);"十二五"国家科技支撑计划重大项目(2012ZX10001-002;2012ZX10001-003;2012ZX10001-004);北京市艾滋病研究重点实验室(BZ0089)~~

摘　　要：目的体外观察人类免疫缺陷病毒-1(human immunodeficiency virus-1,HIV-1)膜蛋白gp120 V3环的神经毒性。方法通过小鼠大脑皮质原代神经细胞培养条件下TUNEL检测和培养上清乳酸脱氢酶释放试验,结合抗微管蛋白-2(microtubule associated protein-2,MAP-2)免疫荧光试验,观察人重组gp120蛋白神经毒性,并通过蛋白印迹法初步探讨其与凋亡调控蛋白bax与bcl-2的关系。结果重组人gp120 V3环多肽可诱导神经元凋亡和抑制神经突起形成,并呈浓度依赖性。gp120 V3环诱导神经细胞凋亡与抑制bcl-2表达有关。结论 HIV-1膜蛋白gp120 V3环具有神经毒性。Objective To investigate the neurotoxicity of human immunodeficiency virus-1 （ HIV-1 ） envelope protein gpl20 in vitro. Methods Primary mouse cortical neuronal cultures were firstly established for neurotoxicity analysis. Terminal dUTP nick end-labeling （TUNEL） assay and lactate dehydrogenase （LDH） leakage assay were used to analyze neuronal apoptosis induced by recombinant gpl20 V3 loop polypeptide. Further, Western blotting was used to detect the levels of apoptosis associated proteins bcl-2 and bax under gpl20 V3 loop exposure. Results Recombinant gpl20 V3 loop polypeptide was found to induce neuronal apoptosis and inhibit neurite outgrowth in a concentration dependent manner, which was associated with low expression of bcl-2 proteins. Conclusion HIV-1 envelope protein gpl20 has neurotoxicity.

关 键 词：膜蛋白gp120 神经毒性 V3环 凋亡 

分 类 号：R512.91[医药卫生—内科学]