男男同性恋人群HIV-1早期感染者T细胞活化水平分析  

Analysis of T cell activation in early HIV-infected individuals from MSM population

作　　者：李珍[1,2] 吴昊[1,2] 张彤[1,2] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心北京市艾滋病研究重点实验室 [2]北京市艾滋病临床研究中心,北京100069

出　　处：《首都医科大学学报》2014年第1期92-95,共4页Journal of Capital Medical University

基　　金："十二五"国家科技支撑计划重大项目(2012ZX10001-006);北京市艾滋病研究重点实验室(BZ0089)~~

摘　　要：目的评价人类免疫缺陷病毒(human immunodeficiency virus,HIV)早期感染者T细胞活化水平,寻找HIV早期感染者疾病进展的预测指标。方法以2010年1月至2010年12月间首都医科大学附属北京佑安医院男男同性恋(men who have sex with men,MSM)队列筛查出的25例HIV早期感染者为研究对象。取冻存的HIV早期感染者和未感染者的外周血单个核细胞(peripheral blood mononuclear cells,PBMCs),流式细胞术检测CD4+T细胞、CD8+T细胞表面活化标志CD38和人类白细胞抗原DR(human leukocyte antigen DR,HLA-DR)的表达。Pearson相关法分析HIV早期感染者CD4+T细胞、CD8+T细胞活化与CD4细胞数、血浆病毒载量的相关性。结果与未感染者相比较,HIV早期感染者HLA-DR+CD4+T细胞、CD38+HLA-DR+CD4+T细胞的比例显著增加(P<0.05),而CD38+CD4+T细胞的比例无明显变化。HIV早期感染者CD38+CD8+T细胞、HLA-DR+CD8+T细胞和CD38+HLA-DR+CD8+T细胞的比例较未感染者均显著增加(P<0.05)。然而,感染时间不同的HIV早期感染者之间相比,CD4+T细胞和CD8+T细胞的活化水平差异无统计学意义。此外,CD38+CD8+T细胞、CD38+HLA-DR+CD8+T细胞的比例与CD4细胞数呈显著负相关,而与血浆病毒载量呈显著正相关。结论 CD38+CD8+T细胞、CD38+HLA-DR+CD8+T细胞的比例有可能作为HIV早期感染者疾病进展预测的指标。Objective To evaluate the immune activation of T cells in early human immunodeficieucy virus （HIV）-infected individuals, to find out the predictors for disease progression of early HIV-infected individuals. Methods Twenty-five early HIV-infected individuals, screened from the cohort of men who have sex with men （MSM） of Beijing You＇An Hospital were enrolled in this study. Frozen peripheral blood mononuelear cells （PBMCs） from early HIV-infected and uninfected individuals were obtained. The expression of activation markers CD38 and human leukocyte antigen DR （HLA-DR） on T cells were detected by flow cytometry. Correlations between T cell activation and CD4 T cell count or plasma viral load in early HlV-infected individuals were analyzed by Pearson test. Results Compared with uninfected individuals, the frequencies of HLA-DR ＋ CD4 ＋ T cells and CD38 ＋ HLA-DR ＋ CD4 ＋ T cells were significantly increased in early HIV-infected individuals （ P 〈 0.05 ）, but no significant difference in the frequency of HLA-DR ＋ CIM ＋ T cells was observed. Moreover, the frequencies of CD38 ＋ CD8＋ T cells, HLA-DR＋ CD8 ＋ T cells and CD38 ＋ HLA-DR ＋ CD8 ＋ T cells were significantly elevated in early HIV-infected individuals compared to uninfected individuals （ P 〈 0. 05 ）. However, no significant differences in CD38 and HLA-DR expression on T cell were observed between early HIV-infected individuals with different infection time. Furthermore, the frequencies of CD38 ＋ CD8 ＋ T cells and CD38 ＋ HLA-DR ＋ CD8 ＋ T cells were negatively correlated with CD4 cell count, and positively correlated with plasma viral load. Conclusion The frequencies of CD38 ＋ CD8 ＋ T cells and CD38 ＋ HLA-DR ＋ CD8 ＋ T cells may be used as the predictor for disease progression in early HIV-infected individuals.

关 键 词：男男同性恋 T细胞 人类免疫缺陷病毒 免疫活化 

分 类 号：R512.91[医药卫生—内科学]