黄芪甲苷和p38MAPK抑制剂对镉致大鼠睾丸损伤的保护效应  

Protective effect of astragaloside Ⅳ and p38MAPK inhibitor on cadmium toxicity on testes in rats

作　　者：宁巍[1] 廖晓岗[1] 姚志勇[1] 王红[1] 范京川[1] 王毅[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016

出　　处：《解剖学杂志》2014年第1期30-33,78,F0004,共6页Chinese Journal of Anatomy

摘　　要：目的：比较黄芪甲苷和p38MAPK抑制剂（SB203580）对镉致大鼠睾丸血睾屏障破坏及相关蛋白表达改变的保护效应。方法：雄性SD大鼠随机分为对照组、单纯镉组、镉加黄芪甲苷组和镉加SB203580组。取睾丸做H-E染色、免疫组织化学和图像分析以及血睾屏障超微结构观察。结果：H-E染色对照组支持细胞核染色较浅且不规则，镉组支持细胞内有空泡形成，镉加黄芪甲苷组与镉加SB203580组未见明显形态异常。免疫组织化学显示对照组波形蛋白阳性产物在支持细胞基室腔附近的胞质中表达，Cx43阳性产物在血睾屏障紧密连接处表达。镉组波形蛋白和Cx43阳性产物表达均显著降低，镉加黄芪甲苷组阳性产物表达低于对照组但明显高于镉组，镉加SB203580组结果与镉加黄芪甲苷组类似。超微结构观察，对照组血睾屏障紧密连接形态完整，呈连续的电子密度较深致密线，镉组血睾屏障紧密连接及支持细胞均见不同程度破坏，镉加黄芪甲苷组与镉加SB203580组破坏程度较相应镉组为轻。结论：黄芪甲苷和p38MAPK抑制剂对镉致大鼠血睾屏障破坏及相关蛋白表达改变具有相似的保护作用。Objective： To compare the protective effect of astragaloside Ⅳ （A） and p38MAPK inhibitor （SB203580） against cadmium （Cd） toxicity on the damage of bloo-testis barrier （BTB） and the alternation of associated protein expression in rats. Methods： SD male rats were randomly divided into 4 groups： control, Cd, Cd＋A and Cd＋SB203580. The testes were studied by light and electron microscopy, immunohistochemistry of vimentin ＆ connexin 43 （Cx43） and semiquantitatively image analysis. Results： In the control group, irregular and lightly stained nucleus of Sertoli cell （Sc） in seminiferous tubules were observed by HE staining. The positive product of vimentin was localized mainly in cytoplasm of Sc near the basal compartment of seminiferous tubules, but the positive product of Cx43 was localized mainly in adjacent Sc membrane regions of tight junction （TJ） formation. A continuous electron density line of TJ and normal ultrastructure of BTB were observed. After Cd treatment, the vesicular formation in the Sc was observed. The average optical density （AOD） of vimentin and Cx43 was decreased significantly compared with that of the control group. The ultrastructural damages of Sc and TJ were observed by electron microscopy. After Cd＋A treatment, AOD of vimentin and Cx43 were increased significantly compared with that of the Cd group, and lower than that of the control group. After Cd＋SB203580 treatment, the alternation including the ultrastructure of TJ and AOD of vimentin and Cx43 was similar to that of Cd＋A group. Conclusion： Cd induces the decrease of vimentin and Cx43 expression and injury of ultrastructure of TJ in BTB, and astragaloside Ⅳ （A） and p38MAPK inhibitor have same protective effect.

关 键 词：镉 黄芪甲苷 P38MAPK抑制剂 连接蛋白43 超微结构 

分 类 号：R331.1[医药卫生—人体生理学]