人脐带间充质干细胞移植治疗小鼠慢性输卵管炎  

Effect of Treatment with Human Umbilical Cord Mesenchymal Stem Cell Transplantation for Chronic Salpingitis in Mice

作　　者：李田[1] 李小毛[1] 张琪[2] 何丹[3] 潘藜捷 

机构地区：[1]中山大学附属第三医院妇产科,广东广州510630 [2]中山大学附属第三医院肝移植中心,广东广州510630 [3]中山大学附属第三医院病理科,广东广州510630 [4]中山大学附属第三医院疫苗研究所,广东广州510630

出　　处：《中山大学学报（医学科学版）》2014年第1期25-31,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2008B030301061)

摘　　要：【目的】观察人脐带间充质干细胞(hUC-MSC)移植对小鼠慢性输卵管炎的治疗作用,为慢性输卵管炎的临床治疗提供新策略。【方法】从脐带中分离培养人脐带间充质干细胞,流式细胞术鉴定其免疫表型,并诱导成脂、成骨分化验证其多向分化潜能。通过阴道内接种沙眼衣原体建立小鼠输卵管炎模型,感染后4周随机分成hUC-MSC移植组及对照组。hUCMSC移植组小鼠阴道内接种人脐带间充质干细胞,对照组小鼠阴道内注射等体积PBS。感染后8周,观察小鼠输卵管有无阻塞、积水等慢性输卵管炎表现,病理切片观察组织形态学改变。【结果】分离培养的人脐带间充质干细胞符合间充质干细胞的一般生物学特性。感染后8周,对照组小鼠均出现输卵管阻塞、积水等慢性输卵管炎表现,而hUC-MSC移植组仅2例出现输卵管阻塞及积水,对照组小鼠慢性输卵管炎的发生率明显高于hUC-MSC移植组,差异有统计学意义(P<0.01)。hUC-MSC移植组输卵管病理切片显示,输卵管周围炎症细胞浸润较少。【结论】hUC-MSC移植可明显减少小鼠慢性输卵管炎的发生和减轻其炎症程度,有可能为输卵管性不孕的临床治疗提供新的途径。[Objective] To investigate the effect of human umbilical cord mesenchymal stem cells （hUC-MSC） transplantation treatment on mice chronic salpingitis, and to provide new tactics for clinical therapy of chronic salpingitis. [Methods] hUC-MSCs were isolated from human umbilical cord and cultured in vitro. The phenotypic characteristics of hUC-MSC were determined by FACS and multi-lineage differentiation capacity was confirmed by induced adipogenesis and osteogenasis. The salpingitis models of mice were established by intravaginal inoculation of the Chlamydia trachomatis. All mice were randomly divided into hUC-MSC transplantation group and control group at 4 weeks after infection. Mice of hUC-MSC transplantation group were transplanted by hUC-MSCs through intravaginal inoculation, and control group were injected by PBS. At 8 weeks after infection, all mice were sacrificed to evaluate the status of oviduct occlussion or hydrosalpinx. Besides, histopathological changes of oviduct were examined by light microscopy. [ Results ] hUC-MSC cultured in vitro exhibited general biological properties of mesenchymal stem cells. At 8 weeks after infection, the incidence of salpingemphraxis and hydrosalpinx in control group was obviously higher than that of hUC-MSC transplantation group, and the difference was of statistical significance （P 〈 0.01 ）. Moreover, tubal pathology section showed that inflammatory ceils infiltration was less in hUC-MSC transplantation group than that of control group. [Conclusion] hUC-MSC transplantation could reduce the occurrence of mice chronic salpingitis and improve tubal inflammation, which may serve as a new method for the clinical treatment of tubal infertility.

关 键 词：间充质干细胞 脐带 输卵管炎 

分 类 号：R711.3[医药卫生—妇产科学]