高乌甲素在大鼠中脑导水管周围灰质的镇痛机制  

Analgesic mechanism of lappaconitine in rat midbrain periaqueductal gray

作　　者：肖智[1] 张福安[2] 张兰英[2] 

机构地区：[1]遵义医学院医学与生物学研究中心,贵州遵义563000 [2]遵义医学院第一临床学院,2009级贵州遵义563000

出　　处：《基础医学与临床》2014年第3期324-327,共4页Basic and Clinical Medicine

基　　金：贵州省优秀科技教育人才省长专项资金[黔省专合字(2011)59];遵义医学院大学生创新性实验计划[院发(2012)2906]

摘　　要：目的观察高乌甲素(LA)能否通过上调中脑导水管周围灰质(PAG)中P2X3受体表达和功能,对烧伤疼痛产生镇痛作用。方法建立Ⅱ°烧伤大鼠模型,给予腹腔注射LA 6mg/kg·24h,Von Frey测痛仪检测机械痛阈值(MWT);Western blot检测P2X3受体蛋白表达;观察干预P2X3受体蛋白的表达和功能后对LA镇痛作用的影响。结果大鼠烧伤后MWT下降(P<0.001),经LA治疗后MWT上升(P<0.001),PAG中P2X3受体蛋白表达上调(P<0.001)。下调P2X3受体蛋白表达后减弱LA镇痛作用;P2X3受体激动剂和拮抗剂分别增强和减弱LA的镇痛作用。结论 LA通过上调PAG中P2X3受体表达和增强P2X3受体功能,促进机体内源性镇痛,对烧伤疼痛大鼠产生镇痛作用。Objective To investigate the analgesic effect of lappaconitine （LA） and its supraspinal analgesic mech- anism with rat model. Methods Male Sprague-Dawley rats were divided into 6 groups： normal, burn, burn LA, burn LA plus antisense oligodeoxynucleotide （A-ODN）, burn LA plus A317491 and burn LA plus ATP. The later 4 groups had LA （6mg/kg） administered intraperitoneally. The pain threshold was characterized by the mechanical withdrawal threshold （MWT） testing; The expression of P2X3 receptor protein in the midbrain periaqueductal gray （PAG） was assessed by Western blot analysis. Observation the intra-PAG injection of P2X3 A-ODN, selective an tagonist and agonist of P2X3 receptor on the analgesic effect of LA were performed. Results In burn group, the MWT was decreased while increased in burn LA group. LA induced a significantly upregulated P2X3 receptor pro tein expression in PAG. The antinociceptive effect of LA was attenuated significantly by P2X3 A-ODN. The α, I3-meATP or A317491 significantly enhanced or attenuated analgesic effect of LA, respectively. Conclusions LA produces an antinociceptive effect on burn pain via increases expression and function of P2X3 receptors in the PAG.

关 键 词：高乌甲素 烧伤疼痛 P2X3受体 中脑导水管周围灰质 

分 类 号：R644[医药卫生—外科学]