N-cadherin对大鼠脊髓损伤后移植神经干细胞存活、增殖与迁移的影响  

Effect of N-cadherin on survival,proliferation and migration of neural stem cells transplanted into injured spinal cord of rats

作　　者：颜建辉[1,2] 黄丽娟[2] 杨柳[2] 华力明[2] 

机构地区：[1]湘南学院临床医学系内科教研室,湖南彬州423000 [2]湘南学院附属医院神经内科,湖南彬州423000

出　　处：《基础医学与临床》2014年第3期372-375,共4页Basic and Clinical Medicine

摘　　要：目的探讨神经钙黏蛋白(N-cadherin)对大鼠脊髓损伤(SCI)后移植神经干细胞(NSCs)存活、增殖与迁移的影响。方法 60只SD大鼠随机分为对照组、模型组、移植1组和移植2组。横断T12复制SCI模型。移植1组行原代培养NSCs移植;以lentivirus病毒为载体将N-cadherin转染至原代培养NSCs再植入移植2组。BBB评分评定大鼠神经功能。术后5、10和15 d免疫荧光检测NSCs存活、增殖和迁移。结果模型组BBB评分显著低于对照组(P<0.05);各移植NSCs组BBB评分均高于模型组(P<0.05),但以移植2组评分最高(P<0.05);移植2组各时间点巢蛋白(Nestin)阳性细胞数量均显著多于移植1组(P<0.05),以15 d的数量最多(P<0.05);移植2组各时间点Nestin阳性细胞迁移距离显著长于移植1组。结论 N-cadherin可以促进NSCs的存活、增殖与迁移,以修复SCI大鼠的神经功能。Objective To investigate the effect on survival, proliferation and migration of NSCs transplanted into SCI of rats. Methods Sixty SD rats were divided into control group, SCI group, transplanting group 1 and trans planting group 2. Using lentivirus virus as a vector, N-cadherin was transfected into primary cultured NSCs to im plant in transplanting group 2. Neurological function were evaluated by BBB score. Immumofluorescence was used to detecte the survival,proliferation and migration of NSCs at 5, 10 and 15 d. Results BBB score of SCI group was lower than that of control group. BBB score in two transplanted groups increased significantly （ P 〈 0.05 ） , with most evident in transplanting group 2 （ P 〈 0.05 ）. The number of Nestin-positive cells in transplanting group 2 was more than that in transplanting group 1 at the corresponding time point（P 〈0.05）. The number of Nestin-positive cells at 15 d was the most among three time points （P 〈 O. 05 ）. The immigrating distance in transplanting group 2 was longer（P 〈 0. 05 ）. Conclusions N-cadherin can promote the survival, proliferation and migration of NSCs in SCI rats to repair neurological function.

关 键 词：神经钙黏蛋白 脊髓损伤 神经干细胞 

分 类 号：R651.2[医药卫生—外科学]