三种波长发光二极管光对光老化皮肤的作用研究  

Efficacy of light-emitting diode irradiation at 530 nm, 630 nm and 850 nm in the treatment of photoaged skin

作　　者：吉杰 丁蕙琳 王晓鑫 张玲琳 王宏伟[3] 王佩茹 王秀丽 

机构地区：[1]上海市皮肤病医院,200050 [2]飞利浦中国研究院 [3]复旦大学附属华东医院

出　　处：《中华皮肤科杂志》2014年第3期166-171,共6页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81272990）;上海市自然科学基金（11ZR1432800）

摘　　要：目的探讨并比较530nm、630nm以及850nm发光二极管（LED）光对光老化皮肤的作用。方法选择14例皮肤光老化受试者，随机于其一侧前臂伸侧三个区域，分别给予530、630及850nmLED光照射，每周3次，共12次。对照射部位皮肤进行皮肤镜检查，并比较照射前、照射第2、4和6周皮肤角质层含水量、经皮水分丢失量（TEWL）以及反映皮肤颜色的皮肤亮度（p值）和皮肤黑素指数（MI值）的变化。同时，随机选择5例、5例、4例受试者在试验前和试验第6周分别取530、630及850nmLED光照射皮肤进行组织病理检查。结果经12次630、850nmLED光照射后，经皮肤镜检查，分别有10例、7例受试者皮肤纹理变浅，色素沉着减少；7例受试者530nmLED光照射部位皮肤纹理加深，色素沉着增加。经630和850nmLED光照射后，14例受试者皮肤角质层含水量均明显升高（6周时分别为37．9±7．7和34．5±7．1au，照射前分别为33．1±6．1和32．0±7．0au，照射前后比较，均P〈0．05），TEWL值均明显降低（6周时分别为9．8±2．5和10．9±2．5g·m-2·h-1，照射前分别为14．0±1．8和14．2±2．6g·m-2·h-1，照射前后比较，均P〈0．05），p值与MI值均未发现明显改变（P〉0．05）。530nm组皮肤角质层含水量及TEWL值较照射前差异均无统计学意义（P〉0．05），L＊值显著降低（P〈0．05），MI值明显增高（P〈0．05）。组织病理检查显示，照射前真皮具有典型的光化性胶原纤维、弹性纤维变性损害，照射后3组均出现新生胶原纤维、弹性纤维，排列较照射前整齐致密。结论630和850nmLED光均可以改善光老化皮肤外观、屏障功能，促进胶原纤维和弹性纤维增生和重排，530nmLED光会增加皮肤色素沉着。Objective To evaluate the efficacy of light-emitting diode （LED） irradiation at 530 nm, 630 nm and 850 nm in the treatment of photoaged skin. Methods A total of 14 adults with photoaged extensor forearm were recruited in this study. Three areas were selected at the extensor forearm in each of these subjects and received LED irradiation at 530 nm, 630 nm and 850 nm, respectively, thrice a week for 12 sessions. Dermoscopy was used to visualize these areas before and after the irradiation, the Corneometer CM 825 to measure stratum corneum （SC） hydration, Tewameter TM 300 to determine transepidermal water loss （TEWL）, and CM-2600d spectrophotometer to measure L＊ value and melanin index （MI）. Tissue specimens were obtained from these areas irradiated with LED at 530 nm （n = 5）, 630 nm （n = 5） and 850 nm （n = 4） separately before and 6 weeks after the initial irradiation, and subjected to histopathological examination. Results Both skin texture and pigmentation were improved in 10 and 7 testees after 12 sessions of LED irradiation at 630 nm and 850 nm, respectively, but the skin texture became rougher and pigmentation was increased in 7 testees after LED irradiation at 530 nm. After LED irradiation at 630 nm and 850 nm, the testees experienced an obvious increase in SC hydration （（37.9 ± 7.7） au and （34.5 ,± 7.1 ） au at 6 weeks after the initial irradiation vs. （33.1± 6.1 ） au and （32.0 ± 7.0） au before irradiation, both P 〈 0.05）, but a significant decrease in TEWL value （（9.8 ± 2.5） and （10.9 ± 2.5） gm-2h-1 at 6 weeks after the initial irradiation vs. （14.0± 1.8 ） and （14.2 ± 2.6） g· m-2· h-1 before irradiation）, with no significant changes in L＊ value or MI （both P 〉 0.05）. No evident alteration was observed in SC hydration or TEWL value （both P 〉 0.05）, while the L＊ value significantly decreased （P 〈 0.05） and MI increased （P 〈 0.05） after LED irradiation at 530 nm. Histopathologieal examination showed phot

关 键 词：皮肤衰老 光 皮肤屏障功能 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]