系统性红斑狼疮患者CD4＋T细胞中miR-17和miR-20a的表达及与病情活动的相关性研究  

Expressions of miR-17 and miR-20a in CD4＋ T cells from patients with systemic lupus erythematosus and their correlations with disease activity

作　　者：文韵诗 秦海红[1] 徐金华[1] 

机构地区：[1]复旦大学附属华山医院皮肤科,上海200040

出　　处：《中华皮肤科杂志》2014年第3期172-175,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30972656）;上海市科委基础研究重点项目（12JC1401900）;上海市优秀学术带头人项目（13XD1401300）;上海市自然科学基金（12ZR1404500）

摘　　要：目的探讨miR-17和miR-20a在系统性红斑狼疮（SLE）患者CD4’T细胞中的表达及与疾病活动的相关性。方法应用实时荧光定量聚合酶链反应（PCR）检测SLE患者活动组17例、缓解组13例及健康对照组18例外周血CD4＋T细胞中miR-17和miR-20a的表达水平，并与SLE患者临床指标进行相关性分析。结果SLE患者组30例miR-17的表达水平（0．24±0．08）显著高于健康对照组（0．15±0．06），两组比较，P〈0．05；两组miR-20a的表达水平分别为0．19±0．10和0．12±0．09，两组比较，P〈0．05。SLE活动组、缓解组与健康对照组miR-17表达水平分别为0．27±0．07、0．20±0．05、0．16-I-0．08，活动组高于缓解组（P〈0．05）和健康对照组（P〈0．05）；miR-20a表达水平三组分别为0．22±0．10、0．15±0．07、0．13±0．09，活动组高于缓解组（P〈0．05）和健康对照组（P〈0．05）；缓解组miR-17和miR-20a的表达水平与健康对照组比较差异均无统计学意义（P〉0．05）。SLE患者组miR-17、miR-20a的表达与SLE活动指数（SLEDAI）均呈正相关（r=0．45，P〈0．05和r=0．38，P〈0．05），与抗dsDNA抗体滴度呈正相关（r=0．54，P〈0．01和r=0．46，P〈0．01），而与c3水平呈负相关（r=-0．43，P〈0．05和r=-0．42，P〈0．05）。结论SLE患者外周血CD4’T细胞中miR-17、miR-20a表达上调，且miR-17、miR-20a表达水平与SLE病情活动相关。Objective To investigate the expressions of miR-17 and miR-20a in CD4＋ T cells from patients with systemic lupus erythematosus （SLE） and their correlations with disease activity. Methods CD4＋ T cells were isolated from the venous blood of 30 patients with SLE and 18 healthy human controls. RNA was extracted from the CD4＋T cells, and real-time fluorescence-based quantitative PCR （qPCR） was performed to determine the expression levels of miR-17 and miR-20a. The intergroup comparison of miR-17 and miR-20a expression levels was done using t test and Mann-Whitney test, and the correlations of miR-17 and miR-20a expressions with clinical parameters were assessed using Pearson or Spearman correlation coefficients. Results The patients with SLE showed increased expression levels （2-Δα） of miR-17 and miR-20a compared with the healthy controls （0.24± 0.08 vs. 0.15 ± 0.06 for miR-17, 0.19 ± 0.10 vs. 0.12 ±0.09 for miR-20a, both P 〈 0.05）. The miR-17 and miR-20a expression levels were also significantly higher in patients with active SLE than in those with inactive SLE and the healthy controls （0.27 ± 0.07 vs. 0.20 ± 0.05 and 0.16 ± 0.08 for miR-17, 0.22 ± 0.10 vs. 0.15± 0.07 and 0.13 ± 0.09 for miR-20a, all P 〈 0.05）, but similar between the patients with inactive SLE and healthy controls （both P 〉 0.05）. The expression levels of both miR-17 and miR-20a were positively correlated with SLE disease activity index （SLEDAI） （r = 0.45, 0.38, respectively, both P 〈 0.05） and anti-dsDNA antibody level （r = 0.54, 0.46, respectively, both P 〈 0.05）, but negatively correlated with serum C3 levels （r = -0.43, -0.42, respectively, both P 〈 0.05）. Conclusions The expressions of miR-17 and miR-20a are increased in CD4＋ T cells from patients with SLE, and correlated with the disease activity in SLE.

关 键 词：红斑狼疮 系统性 CD4阳性T淋巴细胞 miR-17 miR-20a 

分 类 号：R593.241[医药卫生—内科学]