季节性流感疫苗对小鼠感染H7N9禽流感病毒的保护效力  

Immune-protective effectiveness of seasonal influenza split vaccine against H7N9 influenza virus infection in mice

作　　者：吕琦[1] 鲍琳琳[1] 许黎黎[1] 邓巍[1] 李枫棣[1] 袁静[1] 于品[1] 姚艳丰[1] 秦川[1] 

机构地区：[1]中国医学科学院北京协和医学院医学实验动物研究所卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国实验动物学报》2014年第1期44-47,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：十二五科技重大专项(2012ZX10004501-004-003,2012ZX10004501-004-004,2012ZX10004301-8);国家自然科学基金(31370203);科技部H7N9禽流感应急防控专项(KJYJ-2013-01-04)

摘　　要：目的 评价季节性流感裂解疫苗对流感病毒H7N9的免疫保护效力.方法 用我国2012～2013年度季节性流感裂解疫苗,以腹腔注射方式免疫BALB/c小鼠,并设PBS免疫模型组,末次免疫14 d后以5 LD50 A/Anhui/1（H7N9）进行攻试验.感染后观察记录小鼠临床表现,体重变化,并分别于第2天和第4天每组处死3只小鼠,取肺组织和鼻甲骨测病毒滴度和载量.结果 感染后疫苗与模型组小鼠体重下降明显,疫苗组存活率为10%,模型组全部死亡.感染后第4天疫苗组鼻甲骨滴度显著低于模型组.血凝抑制试验及中和实验表明免疫小鼠血清无中和H7N9病毒抗体.结论 季节性流感疫苗在小鼠中对于H7N9流感病毒感染无明显保护作用.Objective To explore whether seasonal influenza split vaccine has immune protective effect on H7N9 influenza virus infection in mice. Methods Seasonal influenza split vaccine and PBS were used to immunize mice by intraperitoneal injection. 14 days later, the mice were booster immunized with A/Anhui/（H7N9） virus. Then the mice were challenged with H7N9 influenza virus in a dose of 5 LD50. H7N9 influenza virus was preincubated with mouse serum immunized with vaccine and titered by micro-neutralization and hemagglutination inhibition （HI） assay. The protective effect was judged by survival rate, body weight loss and residue virus titer in the lungs and turbinates 2 and 4 days post inoculation. Results After infection, the body weight of mice in the vaccine and model groups were declined, the survival rate of the vaccine group was 10% and all mice of the model group died. The virus titers of the turbinates in the vaccine group were significantly higher than that in model group. The neutralizing antibodies raised in the sera of all mice vaccinated with seasonal influenza split vaccine did not neutralize H7N9. Conclusion Seasonal influenza split vaccine cannot effectively protect mice against H7N9 influenza virus infection.

关 键 词：H7N9流感病毒 季节性流感疫苗 免疫保护效力 

分 类 号：Q95-33[生物学—动物学]