雌激素诱导SD大鼠前列腺炎与内环境改变的关系  

Correlation of estrogen-induced SD rat models of prostatitis with changes of internal environment

作　　者：周莉[1] 骆永伟[1,2] 王永[1] 王蓉[1] 刘向云[1] 桂博[1] 贾玉玲[1,2] 孙祖越[1] 

机构地区：[1]上海市计划生育科学研究所药理毒理学研究室,中国生育调节药物毒理检测中心,上海200032 [2]复旦大学药学院,上海201203

出　　处：《中国比较医学杂志》2014年第2期33-37,I0002,共6页Chinese Journal of Comparative Medicine

基　　金：十二五"重大新药创制"科技重大专项(2011ZX09301-005);上海市人才发展基金(2010031);上海市实验动物创新行动计划项目(11140901300/11140901303))

摘　　要：目的通过雌二醇诱导SD雄鼠慢性前列腺炎,探讨SD雄鼠的内环境改变的关联性作用,为前列腺炎发生机制和治疗提供一条实验性途径。方法 3%戊巴比妥麻醉,无菌条件下去势手术;术后动物恢复5 d,30只雄鼠按照体重随机分组,每组10只。第6天低剂量组皮下注射0.25 mg/kg的雌二醇,高剂量组皮下注射1.25 mg/kg的雌二醇,溶媒对照组注射橄榄油,每天1次,连续30 d。结果与溶媒对照组比较,低、高剂量组白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)和红细胞压积(HCT)均降低(P<0.01);前列腺酸性磷酸酶(PAP)明显降低(P<0.01);高剂量组睾酮含量明显增加(P<0.01),C-反应蛋白(CRP)在低、高剂量组也呈现增加的趋势,但差异没有统计学意义;溶媒对照组和给药组前列腺组织大体解剖表面未见明显差异,光学显微镜下,溶媒对照组前列腺组织结构完整、腺腔和间质无炎性细胞浸润,高剂量组和低剂量组的前列腺间质均可见炎细胞浸润;低、高剂量组大鼠脾脏均可见棕黄色颗粒沉淀,其余脏器无明显改变,胸腺、脾脏及其脏器系数均明显降低。结论根据本实验结果,雌激素引起的慢性非细菌性前列腺炎可导致内环境血液学、血清生化,激素水平、脏器和脏器系数的一系列改变。Objective To explore the correlation of internal environment with estradiol-induced male SD rat models of chronic prostatitis ,and provide an experimental approach for its pathogenesis and treatment researches. Methods Anesthesia with 3% pentobarbital, castration surgery under the sterile condition, and recovery for five days were performed in the experiment. Thirty male SD rats were randomly divided into 3 groups, 10 rats in each group. 0. 25mg/kg estradiol （low dose） and 1.25/kg estradiol （high dose）, respectively, were subcutaneously injected from day 6 for 30 consecutive days. The solvent control group received injection of olive oil in the same period. Results WBC, RBC, HGB and HCT were significantly lower in the estrogen groups than that in the control group （ P 〈 0. 01 ）. PAP was significantly decreased （ P 〈 0. 01 ）. Testosterone was significantly increased in the high-dose group （P 〈0. 01 ）, and C-reactive protein （CRP） also showed an increasing trend in the estrogen groups, but statistically not significant. There was no significant difference in the gross appearance of the prostate among the groups. Histopathological analysis revealed that there were inflammatory infiltration and accumulation of brown particles in the spleen tissue in both the low dose and high dose estrogen groups. There were no significant changes in other organs. Organ and organ coefficiente of the thymus and spleen were significantly reduced. Conclusions Our findings indicate that chronic non-bacterial prostatitis induced by estrogen can lead to a series of changes of the internal environment including the hematology, serum biochemistry, hormone levels, organs and organ coefficient.

关 键 词：雌激素 前列腺炎 大鼠 

分 类 号：R33[医药卫生—人体生理学]