PSGL-1基因敲除小鼠血中MIP-1γ和TNF-α的表达  

Expression of MIP-1γ and TNF-α in PSGL-1 knockout mice

作　　者：李江超[1] 周泽启 叶杰[1] 韩露[1] 叶宇翔[1] 刘影[1] 何晓东[1] 陈卉[1] 袁俏冰 黎帅[1] 张静丽[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广东广州510006

出　　处：《中国比较医学杂志》2014年第2期70-73,78,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然基金(31271455);广东省医学科研基金(A2013312)

摘　　要：目的研究PSGL-1缺失对遗传基因工程小鼠外周血血常规的影响,并检测外周血中炎症因子IGFBP-6、TNF-α和MIP-1γmRNA表达水平。方法用血常规检测方法检测正常C57/BL/6小鼠和PSGL-1基因缺失的基因工程小鼠(PSGL-1-/-小鼠)的外周血中血常规的差异;其次,提取两种鼠的血液的mRNA,逆转录为cDNA,采用real-time PCR方法,检测C57小鼠与PSGL-1-/-小鼠外周血中炎症因子TNF-α和MIP-1γmRNA的差异。结果与对照组C57小鼠相比,PSGL-1-/-基因工程小鼠在12周龄时外周血中中性粒细胞(*P<0.05)、淋巴细胞(**P<0.01)和白细胞细胞(***P<0.001)总数显著增加炎症因子TNF-α以及MIP-1γ表达增加(P<0.05)。结论 PSGL-1的缺失改变了小鼠细胞因子并影响了外周血的血细胞组成,MIP-1γ和TNF-α炎症因子上调,有可能影响了小鼠的免疫功能。Objective To investigate the effect of loss of PSGL-1 on routine blood test results in PSGL-1 knockout （PSGL-1-/-） mice, and to detect the expression levels of cytokines IGFBP-6, TNF-α and MIP-1γ mRNA in their peripheral blood. Methods To detect the differences in routine blood test results of normal C57/BL/6 mice and PSGL- 1 -/- mice. To prepare cDNA from isolated blood mRNA, and to analyze the differences in expression of cytokines TNF-α and MIP-1γ mRNA in peripheral blood of the C57 mice （control） and PSGL-1-/- mice by real-time PCR. Results Compared with the C57 control mice, the 12-week old PSGL-1 -/- mice showed significantly increased neutrophils （P 〈 0.05） , lymphocytes （P 〈 0.01 ） and total leukocyte counts （ P 〈0. 001 ） , and increased expression levels of TNF-α and MIP-1γ mRNA in the peripheral blood （P 〈 0. 05）. Conclusions Our findings suggest that the loss of PSGL-1 changes the cell counts of peripheral blood in mice. The up-regulation of cytokines MIP-13, and TNF-α may influence the immune function of the mice.

关 键 词：PSGL-1 TNF-Α MIP-1γ 基因工程动物 

分 类 号：R33[医药卫生—人体生理学]