Comparative analysis of four essential Gracilariaceae species in China based on whole transcriptomic sequencing  

作　　者：XU Jiayue SUN Jing YIN Jinlong WANG Liang WANG Xumin LIU Tao CHI Shan LIU Cui REN Lufeng WU Shuangxiu YU Jun 

机构地区：[1]CAS Key Laboratory of Genome Sciences and Information,Beijing Key Laboratory of Genome and Precision Medicine Technologies,Beijing Institute of Genomics,Chinese Academy of Sciences [2]Beijing Key Laboratory of Functional Genomics for Dao-di Herbs,Beijing Institute of Genomics,Chinese Academy of Sciences [3]University of Chinese Academy of Sciences [4]Changchun University of Chinese Medicine [5]College of Marine Life Science,Ocean University of China

出　　处：《Acta Oceanologica Sinica》2014年第2期54-62,共9页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract Nos 31140070,31271397 and 41206116;the algal transcrip-tome sequencing was supported by 1KP Project(www.onekp.com)

摘　　要：Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-cies which are important resources for the production of special proteins, phycobilisomes, special carbo-hydrates, and agar in China. In this study, de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time. Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G. lema-neiformis and other three Gracilaria species. Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species. The genes encoding allophycocyanin, phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G. lemaneiformis had close relationship to genus Gracilaria, as well as that within genus Gracilaria, G. chouae had closer relationship to G. vermiculophylla rather than to G. blodgettii. The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae.Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-cies which are important resources for the production of special proteins, phycobilisomes, special carbo-hydrates, and agar in China. In this study, de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time. Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G. lema-neiformis and other three Gracilaria species. Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species. The genes encoding allophycocyanin, phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G. lemaneiformis had close relationship to genus Gracilaria, as well as that within genus Gracilaria, G. chouae had closer relationship to G. vermiculophylla rather than to G. blodgettii. The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae.

关 键 词：Gracilaria chouae Gracilaria blodgettii Gracilaria vermiculophylla Gracilariopsis lemaneiformis transcriptome sequencing PHYCOBILIPROTEIN phylogeny 

分 类 号：Q943[生物学—植物学]