The discovery of archaea origin phosphomannomutase in algae based on the algal transcriptome  

作　　者：FENG Yanjing CHI Shan LIU Cui CHEN Shengping YU Jun WANG Xumin LIU Tao 

机构地区：[1]College of Marine Life Science,Ocean University of China [2]Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism,Zhong Shan School of Medicine,Sun Yat-Sen University [3]CAS Key Laboratory of Genome Sciences and Information,Beijing Key Laboratory of Genome and Precision Medicine Technologies,Beijing Institute of Genomics,Chinese Academy of Sciences [4]Beijing Key Laboratory of Functional Genomics for Dao-di Herbs,Beijing Institute of Genomics,ChineseAcademy of Sciences

出　　处：《Acta Oceanologica Sinica》2014年第2期108-113,共6页海洋学报（英文版）

基　　金：The National High Technology Research and Development Program of China under contract No.2012AA10A406;the National Nat-ural Science Foundation of China under contract Nos 41206116,31140070 and 31271397;Technology Project of Ocean and Fisheries of Guangdong Province under contract No.A201201E03;the Fundamental Research Funds for the Central Universities under contract No.201262003;China Post-doctoral Science Foundation under contract No.2011M501167;the algal transcriptome sequencing was supported by 1KP Project(www.onekp.com)

摘　　要：Phosphomannomutase （PMM;EC 5.4.2.8） is an enzyme that catalyzes the interconversion reaction between mannose-6-phosphate and mannose-1-phosphate. However, its systematic molecular and functional in-vestigations in algae have not hitherto been reported. In this work, with the accomplishment of the 1 000 Plant Project （OneKP） in which more than 218 species of Chromista, including 19 marine phaeophytes, 22 marine rhodophytes, 171 chlorophytes, 5 cryptophytes, 4 haptophytes, and 5 glaucophytes were sequenced, we used a gene analysis method to analyze the PMM gene sequences in algae and confirm the existence of the PMM gene in the transcriptomic sequencing data of Rhodophyta and Ochrophyta. Our results showed that only one type of PMM with four conserved motifs exists in Chromista which is similar to human PMM. Moreover, the phylogenetic tree revealed that algae PMM possibly originated from archaea.Phosphomannomutase （PMM;EC 5.4.2.8） is an enzyme that catalyzes the interconversion reaction between mannose-6-phosphate and mannose-1-phosphate. However, its systematic molecular and functional in-vestigations in algae have not hitherto been reported. In this work, with the accomplishment of the 1 000 Plant Project （OneKP） in which more than 218 species of Chromista, including 19 marine phaeophytes, 22 marine rhodophytes, 171 chlorophytes, 5 cryptophytes, 4 haptophytes, and 5 glaucophytes were sequenced, we used a gene analysis method to analyze the PMM gene sequences in algae and confirm the existence of the PMM gene in the transcriptomic sequencing data of Rhodophyta and Ochrophyta. Our results showed that only one type of PMM with four conserved motifs exists in Chromista which is similar to human PMM. Moreover, the phylogenetic tree revealed that algae PMM possibly originated from archaea.

关 键 词：transcriptomic sequencing CHROMISTA PHOSPHOMANNOMUTASE conserved motif phylogenetic analysis 

分 类 号：Q946[生物学—植物学]