植物生长物质对大花红景天愈伤组织诱导及苗再生的影响  

Effects of Plant Growth Substances on Callus Induction and Shoot Regeneration of Rhodiola crenulata Hook. f. et Thoms.

作　　者：王梦亮[1] 张娜莎[1] 郭婷婷[1] 崔晋龙[1] 

机构地区：[1]山西大学应用化学研究所,太原030006

出　　处：《植物生理学报》2014年第2期192-196,共5页Plant Physiology Journal

基　　金：国家自然科学基金(31270383);高等学校博士学科点专项科研基金(20121401120001);山西大学博士科研启动项目(2011BS01)

摘　　要：以大花红景天植物的茎、叶、芽、种子为材料,采用DPS软件正交设计法,通过添加不同植物生长物质,考察其对大花红景天愈伤组织形成及苗再生的影响,为低海拔条件下建立大花红景天植株再生体系提供数据基础。结果表明:种子和芽是诱导愈伤组织最适宜的外植体;植物生长物质对愈伤组织形成的影响大小为6-BA>KT>2,4-D>NAA>IAA,愈伤组织形成的最佳培养基为MS+6-BA 4.0 mg·L-1+KT 0.1 mg·L-1+NAA 0.1 mg·L-1+IAA 0.2 mg·L-1;不定芽分化的最佳培养基为MS+TDZ 0.5 mg·L-1+NAA 0.5 mg·L-1,诱导率达63.14%,不定芽数平均为11.4。本试验获得的最佳培养基配方,适宜在低海拔条件下进行快速诱导大花红景天的愈伤组织和植株再生。To investigate the effects of different plant growth substances on callus induction and shoot regeneration of Rhodiola crenulata,the infancy stems,leaves,buds and seeds were chosen as test materials.The optimum combination of plant growth substances in MS solid medium for plant regeneration was established by using DPS software designing the orthogonal tests.The results showed that the seeds and buds were the optimal explants;the effect of plant growth substances on callus formation was 6-BA＆gt;KT＆gt;2,4D＆gt;NAA＆gt;IAA,the most effective medium was MS＋6-BA 4.0 mg.L-1＋KT 0.1 mg.L-1＋NAA 0.1 mg.L-1＋IAA 0.2 mg.L-1;the most appropriate medium for shoot regeneration was MS＋TDZ 0.5 mg.L-1＋NAA 0.5 mg.L-1,the induction rate was 63.14%,and the average number of adventitious bud was 11.4.The optimum combinations of plant growth substances in MS solid medium which were investigated in this project were avail to induce the callus and establish shoot regeneration system quickly in low altitude conditions.

关 键 词：大花红景天 组织培养 正交设计 植物生长物质 

分 类 号：S567.239[农业科学—中草药栽培]