组织工程骨膜同种异体体内成骨修复兔肩胛骨缺损的初步研究  

PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT

作　　者：张苍宇[1] 王栓科[1] 任广铁[1] 拓振合[1] 庾佳佳[1] 汪静[1] 安丽萍[1] 马婧琳 赵琳[1] 

机构地区：[1]兰州大学第二医院骨科甘肃省骨与关节疾病研究重点实验室,兰州730030

出　　处：《中国修复重建外科杂志》2014年第3期384-388,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(30973064);国家留学回国人员优选资助课题([2010]343)~~

摘　　要：目的 以兔BMSCs和猪小肠黏膜下层（small intestinal submucosa，SIS）复合构建组织工程骨膜，植入同种异体兔体内修复肩胛骨缺损，探讨组织工程骨膜修复大块不规则骨缺损的可行性。 方法取2周～1月龄新西兰大白兔骨髓分离培养BMSCs，取猪近端空肠制备SIS，两者复合构建组织工程骨膜。取6月龄新西兰大白兔18只制备单侧肩胛骨3 cm × 3 cm次全切骨缺损模型，随机分为实验组和对照组（n=9），分别于缺损处植入组织工程骨膜和单纯SIS。术后观察动物大体情况；8周时处死动物后行X线片观察并按Lane-Sandhu X 线片评分标准评分；标本大体观察后，行HE及Masson染色观察。 结果实验动物术后进食、活动均正常，切口无红肿、渗液。X线片观察示实验组骨缺损区有片状新生骨形成，密度与正常骨相同；对照组骨缺损区无骨形成征象，骨缺损区密度与周围软组织影相似。X线片评分实验组为（6.67 ± 0.32）分，对照组为（0.32 ± 0.04）分，比较差异有统计学意义（t=19.871，P=0.001）。标本大体观察示实验组两端桥接部已形成骨性愈合，部分骨缺损区被新生骨填充；对照组无骨组织生成。HE及Masson染色示实验组骨缺损处有新骨生成，骨组织中可见血管腔及髓腔样结构，未见明显巨噬细胞及淋巴细胞浸润；对照组骨缺损区仅为胶原瘢痕组织和纤维样结构，无骨组织生成。 结论以兔BMSCs和猪SIS构建的组织工程骨膜在同种异体兔体内可以成骨，具有修复大块不规则骨缺损的可行性。Objective To investigate the feasibility of tissue engineered periosteum （TEP） constructed by porcine small intestinal submucosa （SIS） and bone marrow mesenchymal stem cells （BMSCs） of rabbit to repair the large irregular bone defects in allogenic rabbits. Methods The BMSCs were cultivated from the bone marrow of New Zealand white rabbits （aged, 2 weeks-1 month）. SIS was fabricated by porcine proximal jejunum. The TEP constructed by SIS scaffold and BMSCs was prepared in vitro. Eighteen 6-month-old New Zealand white rabbits whose scapula was incompletely resected to establish one side large irregular bone defects （3 cm × 3 cm） model. The bone defects were repaired with TEP （experimental group, n=9） and SIS （control group, n=9）, respectively. At 8 weeks after operation, the rabbits were sacrificed, and the implants were harvested. The general condition of the rabbits was observed; X-ray radiography and score according to Lane-Sandhu criteria, and histological examination （HE staining and Masson staining） were performed. Results After operation, all animals had normal behavior and diet; the incision healed normally. The X-ray results showed new bone formation with normal bone density in the defect area of experimental group; but no bone formation was observed in control group. The X-ray score was 6.67 ± 0.32 in experimental group and was 0.32 ± 0.04 in control group, showing significant difference （t=19.871, P=0.001）. The general observation of the specimens showed bone healing at both ends of the defect, and the defect was filled by new bone in experimental group; no new bone formed in the control group. The histological staining showed new bone tissue where there were a lot of new vessels and medullary cavity, and no macrophages or lymphocytes infiltration was observed in the defect area of experimental group; only some connective tissue was found in the control group. Conclusion TEP constructed by porcine SIS and BMSCs of rabbit can form new bone in allogenic rabbit and has

关 键 词：组织工程骨膜 BMSCS 小肠黏膜下层 骨缺损修复 兔 猪 

分 类 号：R318[医药卫生—生物医学工程]