鸡卵提取物促进293T细胞升高表达多能基因OCT4和NANOG  

Chicken Egg Extracts Promote Increased Expression of Pluripotent Gene OCT4 and NANOG in 293T Cells

作　　者：阮光萍 王金祥 姚翔 庞荣清 蔡学敏 何洁 赵晶 潘兴华 

机构地区：[1]解放军昆明总医院干细胞工程实验室,昆明650032

出　　处：《中国细胞生物学学报》2014年第2期190-194,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31172170);国家重点基础研究发展计划(973计划)(批准号:2012CB518106);中国博士后科学基金特别资助项目(批准号:201104748)资助的课题~~

摘　　要：作者找到一种卵细胞提取物有促进293T细胞表达多能基因的作用,这将在细胞生物学有广泛的应用前景。提取鸡卵清、卵黄和全卵提取物,用于293T细胞的渗透诱导。在诱导后不同时间提取细胞的RNA,检测多能基因OCT4和NANOG的变化。在诱导后10 d提取细胞的DNA,检测OCT4和NANOG基因甲基化位点的变化。卵清、卵黄和全卵提取物具有促进293T细胞生长的作用,三种提取物渗透诱导后的293T细胞OCT4和NANOG基因表达有不同程度的升高。OCT4和NANOG基因发生去甲基化,基因开放表达。鸡卵提取物具有促进293T细胞表达OCT4和NANOG基因的作用,有望成为诱导细胞向多能细胞转化的制剂。We find an egg extract that has the role of promoting 293T cells to express pluripotent genes, and it will have broad application prospects in cell biology. Egg-white, egg-yolk and whole-egg extracts were used for the penetration induction of the 293T cells. At different times after induction, OCT4 and NANOG pluripotent gene changes were detected in cell RNA. Ten days after induction, methylation sites changes of OCT4 and NANOG gene were detected in cell DNA. The egg-white, egg-yolk and whole-egg extracts had the role of promoting the growth of 293T cells. After the three extracts permeated the 293T cells, OCT4 and NANOG gene expressions increased to varying degrees. OCT4 and NANOG genes ap- peared demethylation, and gene expression opened. Chicken egg extracts have the roles to increase OCT4 and NANOG gene expression in 293T cells and may be agents to induce somatic cells into pluripotent cells.

关 键 词：鸡卵提取物 293T细胞 多能基因 甲基化 

分 类 号：Q78[生物学—分子生物学]