白藜芦醇对急性痛风性关节炎的影响  

The effect of resveratrol on acute gouty arthritis

作　　者：王鹏[1] 丁慧[1] 孙晓方[1] 陈颖[1] 姚如永[2] 王颜刚[1] 

机构地区：[1]青岛大学医学院附属医院内分泌科,266003 [2]青岛大学医学院附属医院中心实验室,266003

出　　处：《中华风湿病学杂志》2014年第3期160-163,I0001,共5页Chinese Journal of Rheumatology

摘　　要：目的观察白藜芦醇对急性痛风性关节炎大鼠的影响。方法选取Wista大鼠36只，按随机数字表法分为正常对照组、模型组、秋水仙碱组、白藜芦醇低剂量组、白藜芦醇中剂量组、向藜芦醇高剂量组，各组相应采用0．9％氯化钠注射液、秋水仙碱、白藜芦醇低、中、高剂量灌胃7d（每日1次），模型组及各实验组于灌胃第4天把质量浓度25mg／ml（0．05ml）的尿酸钠混悬液注射到大鼠踝关节腔内，制备急性痛风性关节炎模型，正常对照组大鼠关节腔内注射0．9％氯化钠注射液0．05ml，72h后留取踝关节关节液及关节滑膜，应用ELISA法观察关节液中IL-1β、CXCL10的变化，蛋门印迹法检测NF-κBp65在关节滑膜中的表达。关节滑膜用4％中性甲醛固定待做病理。用方差分析进行数据处理。结果关节液中，低、中、高剂量组IL-1β含量分别为（9．63±0．71）、（7．67±0．48）和（6．66±0．29）pg／ml，与模型组（11．85±0．45）pg／ml比较明显下降（P〈0．05），中高剂量组CXCL10含量分别为（86．8±2．9）和（89．6±5．5）pg／ml，与模型组（110．1±4．0）pg／ml比较明显下降（P〈0．05）。蛋白印迹法结果显示，白藜芦醇可抑制关节滑膜NF—κBp65的表达。病理结果显示，向藜芦醇可减轻急性痛风性关节炎大鼠踝关节组织的水肿和炎性细胞浸润。结论急性痛风性关节炎发病过程中IL-1β，CXCL10明显增高，关节滑膜NF-κBp65表达增多，矗藜芦醇可有效抑制急性痛风性关节炎发作，且该作用呈一定的剂量依赖性。Objective To study the effects of resveratrol on acute gouty arthritis. Methods According to random number table, thirty-six Wista rats were randomly divided into six groups： the control group, the model group, the colchicine treatment group, the high dose resveratrol group, the medium dose resveratrol group, the low dose resveratrol group. They were administrated with normal saline, colchicine suspension, high dose resveratrol, medium dose resveratrol, low dose resveratrol for 7 days once daily. Acute gouty arthritis rats models were established on the fourth day by injecting monosodium urate solution （concentration of 25 mg/ml, 0.05 ml） into the ankle joint, while 0.05 ml normal saline was injected into the joint of the control rats. Seventy-two hours after the model was established, the synovial fluid were collected from ankle joints for the measuring of the level of IL-1β, CXCL10. NF-κB p65 protein levels were assayed with Western blotting. The joint synovia were fixed for histopathological examination with 10% fromaldehyde. Variance analysis were used. Results The values of IL-1β in the low, medium and high doses of resveratrol ,sere （9.63 ±0.71 ）, （7.67 ±0.48） and （6.66 ±0.29） pg/ml respectively, being significantly lower than that in the model group （11.85±0.45） pg/ml （P〈0.05）. The values of CXCL10 in the medium and high doses of resveratrol were （86.8±2.9） and （89.6±5.5） pg/ml respectively, being significantly lower than that in the model group （110.1±4.0） pg/ml （P〈0.05）. The Western blotting resuhs showed that the expression of NF-κB p65 protein in the high dose resveratrol group decreased evidently than that in the model group. The histopathological examination showed that resveratrol might reduce joint edema, decrease inflammatory cells infiltration in acute gouty arthritis. Conclusion The levels of IL- 1β, CXCL10 and the expression of NF-κB p65 protein are elevated in acute gouty arthritis. The resveratrol can effectively control acute gouty a

关 键 词：关节炎 痛风性 NF—κB 白藜芦醇 

分 类 号：R589.7[医药卫生—内分泌]