火棘花挥发油化学成分的GC-MS分析及抗氧化活性研究  

GC-MS Analysis on Chemical Constituents and Antioxidant Activity of Volatile Oil from Pyracantha fortuneana Flowers

作　　者：葛丽娜[1] 韩雪[1] 任珂珂[1] 章甫[1] 彭永宇 毕淑峰[1] 

机构地区：[1]黄山学院生命与环境科学学院,黄山245041

出　　处：《植物研究》2014年第2期276-281,共6页Bulletin of Botanical Research

基　　金：中国博士后科学基金项目(2011M500898);国家级大学生创新计划项目(201210375041)

摘　　要：采用水蒸气蒸馏法提取火棘花挥发油,利用气相色谱—质谱联用技术(GC-MS)分析其化学成分。使用维生素C和BHT为阳性对照,以DPPH自由基、亚硝酸钠清除作用为指标评价挥发油的抗氧化活性。从挥发油中鉴定了77个化合物,占挥发油总量的83.77%,含有多种生物活性成分,以萜类及其含氧衍生物(50.31%)、烷烃(18.52%)、醛(5.54%)为主;挥发油对DPPH自由基、亚硝酸钠有明显的清除作用,清除率为50%时,其体积分别为43.51、79.48μL,样品量与清除率间呈量效关系;挥发油对DPPH自由基的清除效果略低于1 mg·mL-1维生素C,对亚硝酸钠的清除效果优于1 mg·mL-1的BHT。The volatile oil from Pyracantha fortuneana flowers was extracted by watersteam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·and sodium nitrite scavenging capacity with VC and BHT as the positive controls. Seventy-seven compounds, which occupied 83.77% of total constituents and contained many bioactive constituents, were identified. Terpenes and their derivatives （50.31%）, alkanes （18.52%）, and aldehydes （5.54%） were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·and sodium nitrite in a concentration-dependent fashion. When the scavenging rate against DPPH· and sodium nitrite of volatile oil was 50%, the volumes of volatile oil were 43.51 and 79.48 μL, respectively. The scavenging capacity against DPPH· of volatile oil was slightly worse than that of 1 mg·mL-1 Vc and the scavenging capacity against sodium nitrite of volatile oil was better than that of 1 mg·mL-1 BHT.

关 键 词：火棘花 挥发油 化学成分 GC—MS 抗氧化活性 

分 类 号：Q946.89[生物学—植物学]