海岛棉ERF基因克隆及乙烯诱导表达分析  

Cloning and ET Induced Expression Analysis of ERF Gene in Gossypium barbadense

作　　者：刘建光[1] 王永强[1] 张寒霜[1] 杜海英[1] 赵俊丽[1] 郭娴[1] 孟宪鹏[1] 

机构地区：[1]河北省农林科学院棉花研究所,农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室,河北石家庄050051

出　　处：《生物技术》2014年第1期20-23,共4页Biotechnology

基　　金：河北省自然科学基金项目("海岛棉抗黄萎病相关ERF转录因子的克隆;功能验证及转化研究";No.C2011301025)资助

摘　　要：目的:利用同源克隆从海岛棉中克隆了1个新的乙烯应答因子(ethylene-responsive factors ERF)为研究该基因功能奠定基础。方法:利用RNA提取试剂盒提取海岛棉叶片总RNA,采用RACE(rapid amplification of cDNA ends,RACE)技术获得基因全长,利用分子生物学软件进行序列分析,利用q-PCR分析基因在乙烯诱导下的表达情况。结果:cDNA全长序列为926bp(GenBank登录号为:KF555288),开放阅读框为624bp,编码208个氨基酸,命名为GbERFa。乙烯诱导在6h表达量最高。结论:该基因参与乙烯信号代谢途径,在棉花防卫反应中可能起一定的作用。Objective： A novel ERF gene （ ethylene - responsive factors ERF） was cloned from C, ossypium barbadense. Leaves. That is the foundation for the further function study. Method： The total RNA was extracted by RNA Extraction Kit. The full - length gene sequence was obtained using homology - based cloning by RACE （ rapid amplification of cDNA ends, RACE） technology, the sequence was analysised with the molecular biology software, and gene expression was analysised under the ethylene induced using q - RT PCR. Result： The full length cDNA sequence is 926bp（ GenBank Accession was No. JQ659260）. The open reading frame （ ORF ） is 624bp encoding a residue of 208 amino acids, named GbERFa. The gene expression was highest at 6h under the ethylene induced. Conclusion： The gene involved in ethylene signaling pathways, and may play a certain role in cotton defense reactions.

关 键 词：ERF转录因子 海岛棉 信号转导 real—time PCR 乙烯 

分 类 号：Q781[生物学—分子生物学] Q786