人白细胞介素-38的特性分析及B细胞表位的预测  

Characteristic Analysis of Human Interleukin- 38 and Prediction of Its B- Cell Epitope

作　　者：袁仙丽[1] 李明才[1] 李燕[1] 高雪明[1] 高巧艳[1] 

机构地区：[1]宁波大学医学院免疫学研究室,浙江宁波315211

出　　处：《生物技术》2014年第1期41-44,共4页Biotechnology

基　　金：国家自然科学基金项目("阻抑IL-33/ST2信号对慢性阻塞性肺疾病气道炎症的作用及机制";No.81070034);宁波市科技创新团队项目("宁波市消化系统恶性肿瘤诊治新技术创新团队";2011B82014);浙江省教育厅科研项目(Y201327689);宁波大学学科项目(XKL11D2112;XKL11D2113)资助

摘　　要：目的:为进一步了解人白细胞介素(IL)-38蛋白质的特性和B细胞表位,从GenBank中查得其序列,用生物信息学等方法预测人IL-38蛋白质的二级结构等特性,并对其B细胞表位进行预测。方法:应用ProtParam tool、ProtScale、Emini和Kolaskar等程序或方法,对IL-38的组成、理化特性、亲水性、柔韧性和可及性等进行分析,以进一步预测其二级结构和抗原表位。结果:IL-38的二级结构主要由无规则卷曲(44.08%)和β-折叠(39.47%)组成。其B细胞表位可能位于第26-36、45-55、78-98和124-143等氨基酸区段。结论:首次利用生物信息学方法对IL-38基因及其蛋白质结构进行了分析和预测,预测结果将有助于确定IL-38的B细胞表位,为IL-38基因功能的深入研究及研制IL-38单克隆抗体提供了理论依据。Objective：Based on interleukin （IL） -38 amino acid sequence from GenBank,the secondary structure and B -Cell epitope were predicted by bioinformaties. Method ：The ProtParam tool, ProtSeale, Emini and Kolaskar were applied to analyze human IL- 38 pro- tein＇ s composition, physical and chemical properties, hydrophileity ,flexibility, aeeessibility, and ordered to forecast seeondary structures, B -cell epitopes. Result：The secondary structure of human IL -38 eontented random coil （44. 08% ） and extended strand （39.47%）. It showed that the most possible epitopes of IL - 38 were located in or adjacent to amino acid 26 - 36,45 - 55,78 - 98,124 - 143. Conclusion：The view was the first report to analysis the construction of protein IL - 38 by bioinformaties. These results would be helpful for the estimate of the epitopes, provided references for further research of human IL - 38 protein function and a theory basis for de- veloping monoclonal antibodies against human IL- 38.

关 键 词：人IL-38 二级结构 B细胞表位 

分 类 号：Q78[生物学—分子生物学] Q518.1