低温氨氮降解菌的筛选及降解能力研究  

Isolation and Ammonium Removal of a Nitrifier at Low Temperature

作　　者：张淑梅[1,2] 姜威[1,2] 李晶[1,2] 孟利强[1,2] 曹旭[1,2] 陈静宇[1] 赵晓宇[1,2] 张云湖[1,2] 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院高技术研究院,黑龙江哈尔滨150001

出　　处：《生物技术》2014年第1期89-92,共4页Biotechnology

基　　金：国家自然科学基金项目("基于异氧硝化菌处理低温微污染水源水中氨氮机理与控制技术研究";No.51078106);黑龙江省重大项目("饮用水安全保障集成技术研究与工程示范";GA13C302);黑龙江省财政基本业务专项("饮用水源氮氮低温降解菌的筛选及菌剂制备")资助

摘　　要：目的:筛选低温高效氨氮降解菌株,探讨其脱氮能力。方法:采用富集培养和纳氏试剂平板显色法分离筛选低温高效氨氮降解菌株;通过形态与生理生化特性、16S rDNA序列分析以及BIOFOSUN微生物鉴定分析系统鉴定菌株,采用液体培养研究菌株的氨氮降解能力和反硝化能力。结果:获得1株低温高效氨氮降解菌株WSW-1001,经形态、生理生化特性、16S rDNA序列以及BIOFOSUN微生物鉴定分析系统鉴定为荧光假单胞杆菌(Pseudomonas fluorescens)。该菌株具有较强硝化和反硝化能力,初始氨氮浓度为5 mg/L,8℃培养24 h,氨氮降解率71.7%,无亚硝酸盐积累。结论:菌株WSW-1001低温氨氮降解能力较强,具有潜在应用价值。Objective： To screen a nitrifier which is capable of removing ammonium at low temperature and study the capability of ammoni- um degradation, Method： The enrichment culture and the Nessler reagent chromogenic method were used to isolate the high effective ni- tfifier capable of degrading ammonium at low temperature. The bacterium was identified by morphological, physiological and biochemical properties and 16S rDNA sequence as well as BIOFOSUN microbial identification analysis. The nitrification and denitrification of the bacte- rium were detected by liquid culture method. Result： A strain WSW - 1001 was isolated from Songhuajiang river, which was identified as Pseudomonas flltorescens on the basis of morphological, physiological and biochemical properties and 16S rDNA sequence as well as BIOFOSUN microbial identification analysis. It carried out nitrification and denitrification and removed 71.7% of ammonium at 8℃ in 24 hours without NO2^＋ -N accumulation. Conclusion： The strain WSW - 1001 was capable of removing ammonium at low temperature and was of practical value in water treatment.

关 键 词：低温 氨氮降解菌 筛选 降解能力 

分 类 号：Q93-331[生物学—微生物学] Q935