白细胞介素-2mRNA在大鼠垂体前叶的表达  

Expression of IL -2 mRNA in rat anterior pituitary

作　　者：韦耿泽[1] 朱运龙[1] 王高峰[1] 韩雪峰[1] 胡玉珍[1] 钟延清[1] 陈健康[1] 

机构地区：[1]第四军医大学基础部生理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2001年第3期201-203,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 !(3 9870 3 19)

摘　　要：目的　研究白细胞介素 - 2 (IL- 2 ) m RNA在正常大鼠垂体前叶的表达 .方法　从正常大鼠垂体前叶组织和离体原代培养的细胞中提取总 RNA,采用二步法 RT- PCR技术 ,扩增出相应大小的 DNA片段 ,将此片段通过凝胶电泳回收纯化后与 p GEM- T easy载体连接 ,转染到大肠杆菌细胞内 ,并接种于 L B培养基中培养 10～ 14h,挑选出阳性克隆进行 DNA序列测定 .结果　测定的 DNA与 IL- 2基因片段相同 .因而 ,在正常大鼠垂体前叶组织和培养细胞中都有 IL - 2 m RNA的表达 .结论　首次证实了 IL - 2不仅存在于垂体肿瘤组织中 ,而且在正常垂体组织中也有表达 .AIM To study the expression of IL 2 mRNA in rat normal anterior pituitary. METHODS Total RNA was extracted from rats' normal anterior pituitary tissues and primary cultured cells in vitro . Using the two step RT PCR technique, we amplified a DNA fragment in comparatively the same length as that of IL 2 mRNA. Through DNA agarose gel electrophoresis, the fragment was purified and then connected with pGEM T easy plasmid by the T4 ligase. The connected plasmid was transformed into the E.coli cells.The transformed cells were planted in the Amp+ LB culture medium and cultured for 10~14 h. The positive clones were selected and then the DNA sequencing was conducted. RESULTS The DNA sequence was the same as that of the IL 2 gene fragment. Therefore, there existed IL 2 mRNA in rats' normal anterior pituitary tissues and cultured cells. CONCLUSION It is proved, for the first time, that IL 2 not only exists in the tissues of pituitary adenoma, but is also expressed in the normal pituitary tissues.

关 键 词：白细胞介素-2 垂体前叶 逆转录-聚合酶链反应 MRNA 

分 类 号：Q453[生物学—生理学]