星点设计-响应面法对石墨炉原子吸收法测定小鼠睾丸组织中镉实验条件的优化研究  

Optimization for determination of cadmium in testis of mouse by graphite furnace atomic absorption spectrometry with central composite design-response surface

作　　者：王梅[1] 徐英[1] 冯乐[1] 陈漫霞[1] 颜戊利[1] 杨冰仪[1] 

机构地区：[1]广东药学院公共卫生学院广东省分子流行病学重点实验室,广东广州510310

出　　处：《环境与健康杂志》2014年第1期54-56,共3页Journal of Environment and Health

基　　金：广东省中医药管理局项目(20122111)

摘　　要：目的应用星点设计-响应面法优化石墨炉原子吸收测定小鼠睾丸组织中镉的实验条件。方法以混合基体改进剂用量（0.3-3．7ml）、灰化温度（252～588℃）和原子化温度（1464～2136℃）为考察对象，以吸光度值作为响应值，进行3因素5水平星点设计，建立数学模型，通过响应面法预测最佳测定条件，并对其进行验证。结果响应值与因素之间可采用二项式拟合，决定系数为0．9851。采用优化的条件（灰化温度为400℃，原子化温度为2020℃，混合基改加入量为2．4m1）测定镉，实测值与预测值仅有1．3％的差别。在此条件下，测定镉的线性范围为0．5～6．0μg／L，RSD为1．19％，检出限为0．136μg／L，平均加标回收率为98．9％。结论星点设计-响应面优化法可用于石墨炉原子法测定条件的优化，所建立的数学模型预测性良好。Objective To employ response surface method based on central composite design to optimize deterrnination condition of graphite furnace atomic absorption spectrometry （GF-AAS） for the determination of cadmium in testis of mouse. Methods Dosage of mixed matrix modifier （0.3-3.7 ml）,ashing temperature（252-588 ℃） and atomization temperature （1 464-2 136 ℃） were selected as the influencing factors and the response value was absorbanee. The experiment mathematical model was arranged according to four-factor, five-level central composite design,and the optimal conditions were predicted by response surface method. Results Absorbance was simulated by using second-order polynomial equation and the regression coefficient was 98.51%. The results showed that the optimum extraction conditions were as follows： ashing temperature was 400 ℃, atomization temperature was 2 020 ℃ and dosage of matrix modifier was 2.4 ml. The experimental value had 1.3% difference with the predicted value under the optimum conditions. A good linearity was obtained for cadmium in the range of 0.5-6.0 μg/L with the lower limit of detection of 0.136 μg/L and the relative standard deviation （RSD） of 1.19%. The average recovery was 98.9%. Conclusion Central composite design and response surface method can be used to optimize test condition of GF-AAS and the model developed in this study has good predictability.

关 键 词：镉 石墨炉原子吸收法 星点设计-响应面法 

分 类 号：O657.6[理学—分析化学]