复方柔肝中姜黄素类成分大鼠药代动力学特征  

Pharmacokinetic characteristics of curcumins in compatibility of Rougan in rats

作　　者：任浩[1] 宿树兰[1] 管汉亮[1] 钱叶飞[1] 钱大玮[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210023

出　　处：《中成药》2014年第3期498-503,共6页Chinese Traditional Patent Medicine

摘　　要：目的 研究复方柔肝（黄芪、姜黄、丹参和熊胆粉）中姜黄素和双去甲氧基姜黄素在大鼠体内药代动力学特征.方法 将SD大鼠随机分为3组,分别灌胃姜黄素和双去甲氧基姜黄素混合物、姜黄提取物、复方柔肝提取物,并采集血浆样本.利用UPLC-TQ/MS测定大鼠血浆中姜黄素和双去甲氧基姜黄素,以多反应监测方式进行正离子检测,应用DAS 3.2计算并比较各组药动学参数.结果 姜黄素在姜黄素和双去甲氧基姜黄素混合物、姜黄提取物和复方柔肝提取物中T1/2、Tmax并无显著性差异,而复方柔肝提取物中Cmax、AUC0～T明显高于姜黄提取物、姜黄素和双去甲氧基姜黄素混合物,姜黄素在姜黄提取物和复方柔肝提取物中MRT0～T略小于姜黄素和双去甲氧基姜黄素混合物,双去甲氧基姜黄素Lmax其他药动学参数均无显著性差异.结论 复方柔肝配伍显著提高姜黄素Cmax,增加了其生物利用度,并加快双去甲氧基姜黄素体内吸收速度,但对其生物利用度影响不大.AIM To study pharmacokinetic of curcumin and bisdemethoxycurcumin from compatibility of Rou- gan （Astragali Radix, Curcumae longae Rhizoma, Salviae mihiorrhizae Radix et Rhizoma, bears gall powder） in rats. METHODS Rats were divided into three groups at random, being respectively fed with the mixture of cur- cumin and bisdemethoxycurcumin （HH）, curcumin extract （TT） and Rougan extract （FF）. Plasma samples of each group were collected. The contents of curcumin and bisdemethoxycurcumin were measured by UPLC-TQ/MS and they were tested by multiple reaction monition in positive ion. The pharmacokinetic parameters of each group were calculated by DAS 3.2 and compared with each other. RESULTS There were no significant differences be- tween groups in TI/2, T-max ; while the C-max and AUG0 -T in FF group were obviously more than these in the TT and HH groups. The MRT0 - T of curcumin in TT and FF goups was slightly smaller than that in the HH group. Expect T there were no obvious differences among pharmacokinetic parameters of bisdemethoxycurcumin. CONCLU- SIONS Rougan decoction can increase C-max and bioavailability of curcumin, and speeds the absorption velocity of bisdemethoxycurcumin hut can not change the bioavailability.

关 键 词：姜黄素 双去甲氧基姜黄素 药代动力学 复方柔肝 

分 类 号：R969.1[医药卫生—药理学]