金钱白花蛇商品药材的高效毛细管电泳指纹图谱研究  

HPCE fingerprint of Bungarus Parvus

作　　者：许靖[1] 王成芳[2] 杜树山[2] 孟繁蕴[2] 李峰[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]北京师范大学中药资源保护与利用北京市重点实验室,北京100875

出　　处：《中成药》2014年第3期563-566,共4页Chinese Traditional Patent Medicine

基　　金：国家自然基金资助项目(30973881);高等学校博士学科点专项科研基金项目(博导类)(20122133110002)

摘　　要：目的建立10批金钱白花蛇药材的毛细管电泳指纹图谱。方法超纯水超声提取,以50 mmol/L硼砂-200mmol/L硼酸(pH8.64)作为背景电解质缓冲液,分析在Beckman P/ACE System MDQ高效毛细管电泳仪上进行,柱温25℃,分离电压20 kV,检测波长245 nm,20 min完全分离。结果建立的指纹图谱有9个共有峰,且方法学考察达到标准。结论金钱白花蛇HPCE指纹图谱与蕲蛇和乌梢蛇有明显差别。AIM To establish a high-performance capillary electrophoresis （HPCE） method for fingerprint of Bungarus Parvus collected from ten different sources. METHODS 50 mmol/L borax-200 mmol/L borieacid buff- er solution （pH8.64） was used for electrolyte buffer and the analysis was performed on Beckman P/ACE System MDQ at column temperature of 25 ℃, isolating voltage 20 kV, and the detection wavelength of 245 nm. RE- SULTS Nine fingerprint peaks were obtained, and the validation of the method met the requirements for SFDA＇ S technical regulations. CONCLUSION The high-performance capillary eleetrophoresis fingerprint shows that the eleetrophoretogram of Bungarus Parvus has a distinct diffenee from that of Agkistrodon and Wushaoshe.

关 键 词：金钱白花蛇 毛细管电泳 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]