毛细管电泳法同时测定清肺抑火丸中5种蒽醌类成分的含量  

Simultaneous Determination of 5 Anthraquinones in Qingfei Yihuo Pills by Capillary Electrophoresis

作　　者：陈岑[1] 王晓可[1] 毋福海[1] 

机构地区：[1]广东药学院,广州510224

出　　处：《中国药房》2014年第12期1118-1121,共4页China Pharmacy

基　　金：广东省自然科学基金资助项目(No.5002841)

摘　　要：目的：建立同时测定清肺抑火丸中5种蒽醌类成分大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚含量的方法。方法：采用毛细管电泳法，以对乙酰氨基酚为内标。毛细管柱为未涂层弹性融硅石英毛细管柱，运行缓冲液为25mmol／L硼砂溶液＋20mmol／L十二烷基磺酸钠＋4mmol／L磺丁基醚．伊环糊精＋2％甲醇（pH10．01），分离电压为18kv，进样方式为重力进样10s（高度15cm），检测波长为254nm，温度为25℃，湿度为〈70％。结果：大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚检测质量浓度分别在5．0～40．0、2．4～19．2、3．6～28．8、11．2～89．9、2．5～20．0μg/ml范围内同各自峰面积与内标峰面积比值呈良好的线性关系（r=0.9980、0．9971、0．9969、0．9985、0.9983）；精密度、稳定性、重复性试验的RSD≤2．40％；平均加样回收率分剐为96．6％、99．6％、99．2％、98．2％、98．6％，RSD分别为0．80％、1．32％、2．21％、1．63％、2．02％（n=6）。结论：该方法专属性强，结果准确、可靠，重复性好，可用于清肺抑火丸的质量控制。OBJECTIVE： To establish a method for simultaneous determination of 5 anthraquinones in Qingfei yihuo pills, such as emodin, aloe-emodin, rhein, chrysophanol and physcion. METHODS： Capillary electrophoresis was adopted using paracetamol as internal standard. The separation was performed on a uncoated fused silica capillary. 25 mmol/L Na2B4O7＋20 mmol/L SDS ＋4 mmol/L SBE-β-CD＋2% methanol solutionfpll 10.01）was selected as the running buffer. The voltage applied was 18 kV. The sample was injected by gravity （1 0 s, 15cm）. The detection wavelength was set at 254 nm. The column temperature was 25 ℃ and humidity was lower than 70%. RESULTS： The linear range of emodin, aloe-emodin, rhein, chrysophanol, physcion were 5.0-40.0 μg/ml（r=0.998 0）, 2.4-19.2 μg/ml（r=0.997 1）, 3.6-28.8μg/ml（r=0.996 9）, 11.2-89.9 μg/ml（r=0.998 5）, 2.5-20.0 μg/ml （r=0.998 3） , respectively; RSDs of precision, stability and reproducibility tests were all lower than 2.40%; average recoveries were 96.6% （RSD=0.80%, n=6）, 99.6% （RSD=1.32%, n=6）, 99.2% （RSD=2.21%, n=6）, 98.2% （RSD=1.63%, n=6） and 98.6% （RSD=2.02%, n=6）. CONCLUSIONS： The method is proved to be specific, accurate, reliable and reproducible, and can be used for quality control of Qingfei yihuo pills.

关 键 词：毛细管电泳法 清肺抑火丸 大黄素 芦荟大黄素 大黄酸 大黄酚 大黄素甲醚 含量测定 

分 类 号：R917[医药卫生—药物分析学]