大鼠胰腺促性腺激素释放激素受体mRNA的原位杂交  

In situ hybridization of gonadotropin releasing hormone receptor mRNA in rat pancreas

作　　者：蒲若蕾[1] 黄威权[1] 姬秋和[1] 姚兵[1] 孙岚[1] 张荣庆[2] 王雷[2] 

机构地区：[1]第四军医大学基础部组织胚胎学教研室,陕西西安710033 [2]清华大学生物系,北京100084

出　　处：《第四军医大学学报》2001年第1期8-11,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目! (39770 388)

摘　　要：目的　研究大鼠胰腺促性腺激素释放激素 (Gn RH)受体 m RNA的存在及细胞定位 .方法　采用原位杂交组织化学法 .结果　胰脏的外分泌部腺上皮细胞、胰导管上皮细胞及内分泌部胰岛细胞内均可检测到较强的 Gn RH受体m RNA杂交信号 .杂交阳性信号物质均分布在胞质内 ,胞核呈阴性 .结论　大鼠胰脏内、外分泌部均能合成 Gn RH受体 .Gn RH也是一种消化激素 ,它由胰脏自身合成 ,又作用于胰脏 ,可能参与胰脏内。AIM To study whether gonadotropin releasing hormone receptor (GnRHR) mRNA exists in rat pancreas and its cellular localization. METHODS In situ hybridization with digoxigenin labeled oligonucleotide probes was adopted. RESULTS Pancreatic endocrine islet cells, exocrine glandular cells and exocrine duct epithelial cells were all found to have GnRHR mRNA hybridization signals. The signals distributed in cytoplasm of all positive cells with negative nucli . CONCLUSION Both of the exocrine and endocrine cells of the pancrea can produce GnRH recepor. GnRH is also a kind of digestive hormone produced by pancrea and is responsible for modulating the exocrine and endocrine function.

关 键 词：促性腺素释放激素 胰腺 受体MRNA 原位杂交 

分 类 号：R335[医药卫生—人体生理学] R333.3[医药卫生—基础医学]