IL-1β加酵母RNA对照射小鼠外周血肝脾GSH与SOD活性的影响  

IL-1β with yeast RNA affects activity of glutathione and superoxide dismutase in blood lever and spleen in mice after γ-irradiation

作　　者：田芙蓉[1] 曾桂英[1] 谷小刚[2] 

机构地区：[1]第四军医大学预防医学系放射医学教研室,陕西西安710033 [2]第四军医大学预防医学系毒理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2001年第1期51-53,共3页Journal of the Fourth Military Medical University

基　　金：第四军医大学"2 11"工程专项经费资助项目!(98X2 0 7)

摘　　要：目的　研究 IL- 1β(人重组白介素 - 1)复合酵母 RNA对小鼠受 γ射线外照射后外周血、肝、脾谷胱甘肽 (GSH)和超氧化物歧化酶 (SOD)活性的影响 ,探讨药物治疗辐射损伤机制 .方法　雌性小鼠 2 5只 ,随机分为 5组 ,115 0 c Gy腹部照射后 1h,2 4h分别给予后肢 im IL - 1β,酵母 RNA,IL - 1β复合酵母 RNA,于照后 6 d,取外周血、肝脏和脾脏测 SOD,GSH活性 .结果　照射后外周血、肝、脾 SOD,GSH活性下降 .使用 IL - 1β,酵母 RNA,IL - 1β复合酵母 RNA后照射组外周血、肝、脾 SOD,GSH活性升高 ,且肝、脾的 SOD,GSH活性与照射对照组之间有显著差异 (P<0 .0 5 ) ,复合组肝脾SOD,GSH活性明显高于照射对照组具有显著差异 (P<0 .0 1) ,高于照射单独给药组 .外周血各照射组之间无显著差异 .结论　 IL- 1β复合酵母 RNA升高 γ射线外照射对肝、脾谷胱甘肽 (GSH) ,超氧化物歧化酶 (SOD)活性 ,复合用药可以对抗射线引起机体产生的损伤作用 .结果表明 :照后复合应用 IL - 1β,酵母 RNA可通过提高清除自由基 。AIM To study mechanism of IL 1β with yeastRNA to protect against irradiation. METHODS Twenty five BALB/c female mice were abdominal irradiated with 1150 cGy, and divided into five groups at random: Control (-), control (+), IL 1β, yeast RNA and combining IL 1β with yeast RNA. IL 1β, yeast RNA, IL 1β and yeast RNA were injected intramuscularly into mice 1 or 24 h after 1150 cGy abdominal γ irradiation. The activity of glutathione and superoxide dismutase were measured in different tissues of mice on d 6 after irradiation. RESULTS Activity of glutathione and superoxide dismutase decreased in tissue after irradiation. The superoxide dismutase and glutathione activity increased in liver and spleen if the mice were treated by IL 1β, yeast RNA after irradiation ( P <0.05). In addition, the superoxide dismutase and glutathione activity increased if the mice were treated by combining IL 1β with yeast RNA after irradiation ( P <0.01). CONCLUSION Combining IL 1β with yeast RNA can scavenge free radicals induced by irradiation.

关 键 词：白细胞介素1 酵母 RNA 电离国徽 谷胱甘肽 肝脾 超氧化物歧化酶 

分 类 号：R818.059[医药卫生—放射医学]