蟾酥注射液诱导人膀胱癌细胞T24、BIU-87凋亡的研究  

Apoplosis of bladder cancer cells T24 and NIU-87 induced by venenum bufonis

作　　者：吴江平[1] 张展东[1] 江沂[1] 苏宁[1] 杨振淮[1] 

机构地区：[1]广州市中医医院,510130

出　　处：《国际医药卫生导报》2014年第6期743-746,共4页International Medicine and Health Guidance News

基　　金：广东省《2009年建设中医药强省科研》项目（2009310）

摘　　要：目的验证蟾酥注射液的抗癌活性。方法将T24细胞、BIU-87细胞分别接种于RPMI1640培养液中37℃孵育，并在多个时间点取细胞悬液加入AO／EB染料染色，在荧光显微镜下观察。结果在0、3、6、12、24和48h时，T24细胞的凋亡率分别为3．0％、12．5％、59．5％、66，5％、70．O％和63．5％，BIU-87细胞的凋亡率分别为2．0％、13．5％、55．5％、62．5％、68．5％和64．0％。结论蟾酥注射液可显著诱导T24细胞、BIU-87细胞凋广，在体外具有抗癌活性。Objective To determine Ihe anticaneer activity of venenum bufimis injection. Methods T24 and BIU-87 cells were seeded in RPMI 1640 culture medium and incubated at 37 ℃ . Cell suspension was taken al muhiple time points and stained by AO/EB dye and then was observed under fluorescence microscope. Resuhs At hour 0, 3, 6, 12, 24, and 48. the apoplosis rates of T24 cells were 3.0%, 12.5%, 59.5%, 66.5%. 70.0%. anti 63.5% respectively; anti the apoptosis rates of BIU-87 cells were 2.0%, 13.5%, 55.5%, 62.5%, 68.5%, and 64.0%, respectively. Conclusions The apoplosis ofT24 anti BIU-87 cells can be obviously induced by venenum bufonis injection.Venenum bufi）nis injection has antieancer activity in vitro.

关 键 词：AO EB双荧光染色法 蟾酥注射液 细胞凋亡 

分 类 号：R285[医药卫生—中药学]