TOLL样受体4、过氧化物酶体增殖物激活受体γ在慢性阻塞性肺疾病肺血管重塑的作用  

The role of Toll-like receptor 4 and peroxisome proliferator-activated receptorγ on pumonary vascular remodeling of chronic obstructive pulmonary disease

作　　者：董蕾[1] 张馨文[1] 丛金鹏 于文成[1] 

机构地区：[1]青岛大学医学院附属医院呼吸内科,266000

出　　处：《中国综合临床》2014年第3期280-283,共4页Clinical Medicine of China

摘　　要：目的 探讨过氧化物酶体增殖物激活受体γ（Peroxisome proliferator-activated receptorγ PPAR-γ）及TOLL样受体4（Toll-like receptors TLR4）在慢性阻塞性肺病（COPD）肺血管重塑中的作用及相关性.方法 收集男性鳞癌手术患者90例,根据肺功能分为COPD组和对照组（非COPD组）,各45例,取其肺叶切除后的无肿瘤浸润外周肺组织,HE染色光镜下观察血管炎症程度并测定管壁厚度占血管外径百分比（WT％）、血管壁横断面积占管径总面积百分比（WA％）；应用免疫组织化学方法检测肺动脉内皮细胞PPAR-γ及TLR4的表达情况.结果 对照组及COPD组中反应血管重建的WA％、WT％及肺动脉内皮细胞PPAR-γ、TLR4阳性表达率的比较差异均有统计学意义[WA％：（26.09±2.82）％与（43.98±6.43）％,t=-13.949 P=0.000; WT％：（15.57±1.75）％与（27.37±3.34）％,t=-7.140,P=0.000; PPAR-γ：（28.22±15.08）％与（10.74±8.81）％t =5.483,P =0.000;TLR4：（4.67±4.47）％与（31.41±14.67）％,t=-9.555,P=0.000].PPAR-γ与TLR4表达呈负相关（r=-0.404,P＜0.01）.结论 COPD患者肺动脉炎细胞浸润,血管机化重塑,PPAR-γ、TLR4表达异常均参与COPD肺血管重塑的调控.Objective To explore the role and correlation of peroxisome proliferator-activated receptor γ （PPAR-γ） and Toll-like receptor 4 （TLR4） on pulmonary vascular remodeling of chronic obstructive pulmonary disease（COPD）.Methods Ninety male patients who underwent surgery for squamous cell carcinoma were enrolled as our subjects.All patients were divided into COPD group and uon-COPD group based on lung function,and 45 cases in each group.Peripheral lung tissues without tumor infiltrated after lobectomy were taken to assess the degree of arterial inflammation,percentage of wall thickness to vessel diamater （WT％） and percentage of wall area to total vascular area（WA％） were measured through Hematoxylin-Eosin（HE） staining under light microscope.The expression of PPARγand TLR4 were determined by immunohistochemistry.Results The distribution of WA％ and WT％ in COPD group were （43.98 ±6.43）％ and （27.37 ±3.34）％,higher than that of non-COPD group （（26.09 ± 2.82） ％,t =-13.949,P =0.000 ; （15.57 ± 1.75） ％,t =-7.140,P =0.000）.The expression of PPAR-γand TLR4 in COPD group were （10.74 ± 8.81） ％,（3 1.41 ± 14.67） ％ respectively,and （28.22 ± 15.08）％,（4.67 ± 4.47）％ in non-COPD group.The differences were statistic significantly（t =5.483,P =0.000; t =-9.555,P =0.000）.And there was negative correlation between the expression of PPARγand TLR4 （r =-0.404,P ＜ 0.01）.Conclusion The pulmonary vascular of COPD patients showed the obviously inflammatory cell infiltration,fibrosis and proliferation,and PPAR-γ and TLR4 participate in the regulation of pulmonary vascular remodeling.

关 键 词：慢性阻塞性肺血管重塑 氧化物酶体增殖物激活受体γ TOLL样受体4 

分 类 号：R563.9[医药卫生—呼吸系统]