增龄对大鼠骨骼肌细胞葡萄糖转运蛋白的影响  

Influence of age on content of glucose-transporter 4 in rat skeletal muscle cells

作　　者：吴毅[1] 杨晓冰[1] 李云霞[1] 占飞[1] 胡永善[1] 朱尚权[2] 平蓓芳[2] 

机构地区：[1]复旦大学医学院附属华山医院运动医学科,上海市200040 [2]中国科学院上海生物化学研究所

出　　处：《中华老年医学杂志》2001年第1期41-43,共3页Chinese Journal of Geriatrics

基　　金：本课题受卫生部科研基金资助!(96 1 165 )

摘　　要：目的　探讨增龄对大鼠骨骼肌细胞葡萄糖转运蛋白 4(glucosetransporter 4,GLUT4 )的影响。　方法　SD实验大鼠分为 2组 :青年组 (3月龄 )和老年组 (2 4月龄 )各 10只。制备GLUT4 羧基端12肽的多克隆抗体 ,利用Western印迹法检测 2组大鼠骨骼肌细胞GLUT4 蛋白含量 ,并检测大鼠尾静脉血糖。　结果　老年组大鼠的血糖 (5 6± 0 5mmol/L)略高于青年组大鼠 (4 5± 0 5mmol/L) ,但差异无显著性 ;青年组大鼠骨骼肌细胞内GLUT4 相对含量为 10 9 6 2± 12 2 5 ,而老年组为 86 46± 8 2 5 ,差异有显著性 (P <0 0 5 )。　结论　增龄可引起大鼠骨骼肌细胞GLUT4 蛋白含量明显减少 ,使骨骼肌细胞对葡萄糖的转运发生障碍 ,这可能是老年人易产生胰岛素抵抗的机制之一。Objective To investigate the difference of glucose transporter 4 (GLUT 4) protein content in skeletal muscles of young and aged rats. Methods Twenty SD rats were divided into two groups, namely young rats (3 months) and aged rats (24 months). The content of GLUT 4 protein in skeletal muscle cells was detected by Western blot analysis in two group rats. The polyclonal antibody against GLUT 4 protein was obtained from GLUT 4 protein immunized rabbits. Blood glucose level of two groups was also detected. Results The relative content of GLUT 4 protein in skeletal muscle cells of aged rats (86 46±8 25) decreased 21 1% as compared with young one (109 62±12 25) ( P <0 05). There was no significant difference in blood glucose level between two groups. Conclusions The decreased content of GLUT 4 protein in skeletal muscles cells may associated with age related insulin resistance in aged.

关 键 词：骨骼肌细胞 葡萄糖转运蛋白 胰岛素抵抗 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]