衍生化-液相色谱-紫外检测联用测定尿中γ-羟基丁酸  

Determination of Gamma-hydroxybutyric Acid in Human Urine with Pre-column Derivatization by High Performance Liquid Chromatography-ultraviolet Detection

作　　者：陈学国[1] 马生祥[1] 马玄中 党斌[1] 刘迟[1] 

机构地区：[1]中国刑警学院法化学系,辽宁沈阳110854

出　　处：《福建分析测试》2014年第2期20-22,共3页Fujian Analysis & Testing

基　　金：公安部应用创新项目(项目编号:2010YYCXLNST012);辽宁省自然科学基金项目(No.201102246);大学生创新训练计划项目(No.201210175051)

摘　　要：本文建立了尿中γ-羟基丁酸的水相催化衍生化-液相色谱-紫外检测联用分析方法。尿样中直接加入衍生化试剂α-溴苯乙酮、催化剂四丁基溴化铵,在80℃水浴中加热30 min,衍生化产物直接进行液相色谱-紫外检测。尿中γ-羟基丁酸浓度在0.5μg/mL-250μg/mL之间具有良好的线性关系,最低检出限为0.20μg/mL。该方法具有较好的灵敏度、准确性和精密度,能够满足司法鉴定工作的要求,具有一定的实用性。A method of analysis of gamma-hydroxybutyric acid（GHB）in human urine was presented with pre-column derivatization by high performance liquid chromatography-ultraviolet detection. 2-bromoacetophenone was using as derivative reagent and tetrabutyl ammonium bromide as activator,the derivative of GHB in urine was directly analyzed by high performance liquid chromatography-ultraviolet detection. The experimental results show that the calibration curves showed good linearity in the range of 0.50μg/mL-250.0μg/mL and the limit of detection was 0.20μg/mL with pre-column derivatization and high performance liquid chromatography-ultraviolet detection of GHB in human urine. The results show high accuracy,good precision and sensitivity,and it can be widely applied in the identification of GHB in cases.

关 键 词：法医毒物分析 Γ-羟基丁酸 液相色谱 尿 药物 

分 类 号：O657.72[理学—分析化学]