石油脱硫微生物的筛选及特性研究  

Study on Isolation and Characteristics of a Dibenzothiophene-Desulfurizing Bacteria

作　　者：王哲[1] 

机构地区：[1]山东农业大学资源与环境学院,山东泰安271018

出　　处：《现代农业科技》2014年第4期199-200,203,共3页Modern Agricultural Science and Technology

摘　　要：从泰安某加油站采集土壤样品,筛选到一株可以专一性降解二苯并噻吩(DBT)的菌株,经常规和生理生化鉴定判断为红球菌属(Rhodococcus sp.),命名为YY-5。对DBT的降解产物经分析为二羟基联苯(2-HBP)、二苯并噻砜(DBTO2)和硫酸根离子,完全符合"4S"途径。产物用gibb’s试剂结合分光光度计测试,测算出菌株的脱硫能力。脱硫试验表明,DBT浓度对菌株的脱硫能力也有影响,DBT浓度过高,会抑制菌株的脱硫能力。一般在54 h左右YY-5的酶活性开始降低,在80 h左右完成脱硫。在以正十二烷、正十六烷、液体石蜡、萘和柴油为碳源的培养基中生长不旺盛,不能利用上述烃类作唯一碳源和能源。说明YY-5在脱硫过程中不会明显降低燃油的燃烧值。另外试验也表明,有机氮对菌的脱硫活性有抑制作用。Collecting soil sample from a gas station in Taian City ,a dibenzothiophene-desulfurizing strain was isolated and identified as Rhodococcus sp. which metabolite as dihydroxybiphenyl named YY-5.Testing the metabolite with gibb ’s agentia and spectrophotometer in order to measure the desulfurization ability of the strain.The degradation products of DBT is dihydroxybiphenyl （2-HBP）,dibenzothiophene sulfone （DBTO2） and sulfate ion,which exactly corresponding to the“4S”pathway.In the desulfuration experiment,the DBT concentration affects the desulfurization ability of YY-5.YY-5 could not use n-dodecane ,n-hexadecath ,liquid paraffin ,naphthalene and diesel oil as it ’s carbon source or energy.So YY-5 will not obviously lower down combustion value of the oil.Other experiment showed that organonitrogen would suppress the activity of desulfurization of YY-5.The rest characteristics of the strain will be discovered by more experiments.

关 键 词：二苯并噻吩 微生物脱硫 筛选 红球菌属 脱硫率 RHODOCOCCUS SP 

分 类 号：S182[农业科学—农业基础科学]