滇龙胆不同居群间品质评价及质量等同性的研究  

Quality Evaluation and Quality Equivalence of Gentiana rigescens Franch. from Different Populations

作　　者：张琳[1] 罗智渊[1] 冯丽丽[1] 李海峰[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《中国药学杂志》2014年第5期412-418,共7页Chinese Pharmaceutical Journal

基　　金：云南省教育厅基金重点项目(2013Z153);大理学院2012年应用开发基金资助项目;大理学院2011年中青年学术带头人培养项目

摘　　要：目的建立滇龙胆不同居群间品质评价及质量等同性的研究方法。方法采用高效液相色谱法测定滇龙胆根中活性成分龙胆苦苷、獐牙菜苦苷、獐牙菜苷含量,建立其HPLC指纹图谱,并用单因素方差分析、聚类分析和主成分分析对其品质及质量等同性进行评价。结果大理白族自治州滇龙胆不同居群间整体品质较好,龙胆苦苷含量较低的巍山居群龙胆的龙胆苦苷含量是国家标准的2倍以上,滇龙胆不同居群间根中龙胆苦苷、獐芽菜苦苷、獐芽菜苷含量(P<0.01)具有极显著差异;对不同居群间质量等同性进行分析,确定HPLC指纹图谱中11个共有峰,共有峰HPLC指纹图谱相似度均在0.988以上,指认其中龙胆苦苷、獐牙菜苦苷、獐牙菜苷3个与活性相关的特征峰,不同居群间质量等同性基本一致;不同居群间龙胆苦苷、獐牙菜苦苷、獐牙菜苷总含量聚类分析和HPLC指纹图谱主成分分析结果相似。结论根据有效成分含量及HPLC指纹图谱得出优质种质资源居群为剑川、鹤庆居群;建立滇龙胆不同居群间品质评价及质量等同性的研究方法,为从滇龙胆野生居群中进一步筛选优质种质资源提供理论依据。OBJECTIVE To establish evaluation methods of the quality and quality equivalence of C, entiana rigescens franch. from different populations. METHODS The contents of the active ingredients, gentiopicroside, swertiamarin and sweroside in the roots of Gentiana rigescens Franch. , were determined by HPLC, and the HPLC fingerprint was established. The quality and quality e- quivalence were evaluated by One-way ANOVA, cluster analysis and principal component analysis（PCA）. RESULTS The overall quality of Gentiana rigescens Franch. from dif[erent populations in Dali Yunnan was better. The content of gentiopicroside in the sample from Weishan population was lower than those in the other populations, while it was more than twice the national standard. There was significant difference（P 〈 0. 01 ） in the contents of gentiopicroside, swertiamarin, and sweroside in tile roots of Gentiana rigescens Frauch. from different populations. The quality equivalence of Gentiana rigescerts Franch. from different populations was analyzed, and 11 common peaks in HPLC fingerprint were selected to evaluate the similarities of the crude drugs. The similarities of the characteristic peaks were all above 0. 988. The three characteristic peaks of gentiopicroside, swertiamarin and sweroside which were related to the ac- tivity of Gentiana rigescens Franeh were identified, and the qualities of different populations were basically equivalent. The total con- tents of gentiopicmside, swertiamarin and sweroside of Gentiana rigescens Franch. from different populations were similar as shown by cluster analysis and PCA of HPLC fingerprint. CONCLUSION Jianchuan and Heqing polulations are quality germplasm populations according to the contents of effective components and HPLC fingerprint. The evaluation methods of quality and quality equivalence of Gentiana rigescens Franch. from different populations are established, which would provide the theoretical basis for further filtering of quality germplasm resources of C, entiana rigescens Franch. fro

关 键 词：滇龙胆 高效液相色谱法 含量测定 指纹图谱 

分 类 号：R284[医药卫生—中药学] Q945[医药卫生—中医学]