防感煎剂对H1N1病毒感染小鼠TNF-α、IL-6的影响  

Influence of Fanggan Decoction on TNF-α and IL-6 in Mice Infected by Influenza a Virus(H1N1)

作　　者：徐一凯 杨珺超[2] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]浙江中医药大学附属第一医院,浙江杭州310006

出　　处：《云南中医学院学报》2014年第1期12-14,25,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：浙江省医药卫生科技计划项目(2010KYB078)

摘　　要：目的探讨防感煎剂对H1N1流感病毒感染小鼠TNF-α、IL-6的影响,进一步了解防感煎剂的疗效机理。方法选用清洁级ICR雄性小鼠96只,随机分为4组:正常对照组、模型对照组、防感煎剂组、达菲对照组。实验组予防感煎剂灌胃给药,达菲对照组予达菲灌胃给药,正常对照组和模型对照组则用生理盐水灌胃,在灌胃给药第3天开始用H1N1流感病毒液滴鼻造模,造模后的第1、5、9、13天每组随机处死6只小鼠,收集肺组织标本,用于H1N1病毒核酸提取,收集血清,用ELISA法检测肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量。结果 1、防感煎剂组小鼠一般状况优于模型组。2、防感煎剂组小鼠肺组织H1N1病毒含量低于模型组(P<0.05)。3、防感煎剂组小鼠血清TNF-α、IL-6水平低于模型组(P<0.01)。结论防感煎剂可抑制H1NI病毒复制,抑制细胞因子TNF-α、IL-6的表达,从而减轻由炎性介质过度释放引起的免疫病理损伤,由此推断防感煎剂可能通过调节免疫功能来控制感染、改善症状。Objective To Explore the influence of Fanggan decoction on TNF alpha and IL-6 in the mice infected by influenza a virus （H1N1）, to learn more about Fanggan decoction curative effect mechanism. Methods Choose clean level 96 male ICR mice, were randomly divided into 4 groups：normal control group, model control group, Chinese medicine in Chinese medicine dosage group and positive control group. Sense of Chinese traditional medicine group to prevent decoction medium dose to fill the stomach, positive control group to tamiflu lavage, normal control group and model control group with saline lavage. In for 3 clays with the flu virus droplets nasal H1N1 virus infection of animal model is set up. In building 1, 5, 9, 13 clays after the random death 6 mice in each group, the serum and lung tissue collection, TNF alpha and IL-6, such as secretion and function. Results 1, Fanggan decoction group is better than the model group in general condition of mice. 2, Fanggan Decoction group had lower H1N1 virus content in lung tissue of mice than those in the model group （P〈0. 05）. 3, drug intervention group mice blood serum tumor necrosis factor （TNF alpha）, IL -6, level significantly decreased, compared with the model group had significant difference （P〈0. 01）. Conclusion Fanggan decoction can depress the replication of the H 1NI virus, depress expression of cytokines TNF-α, IL-6, thereby reducing the excessive release of inflammatory mediators by immune pathological injury caused by inflammation, thus Fanggan decoction can rezulate the immune function and imDrove svmntoms.

关 键 词：防感煎剂 甲1型流感病毒 TNF-Α IL-6 

分 类 号：R285.5[医药卫生—中药学]