USPIO增强MR与Gd-DTPA增强MR在兔动脉粥样硬化斑块中的对比研究  

Comparative study of USPIO-enhanced and Gd-enhanced MRI in the diagnosis of atherosclerotic plaques in rabbits

作　　者：王露朝 李公信[1] 刘芃[1] 温志波[1] 黄凡衡[1] 陈立桁 赵鑫[1] 林霖[1] 周怡军[1] 

机构地区：[1]南方医科大学珠江医院心内科,广州市510280

出　　处：《实用医学杂志》2014年第5期684-687,共4页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:S2011010004025)

摘　　要：目的:初步探讨USPIO(羧基化三氧化二铁磁性纳米颗粒)增强MR与Gd-DTPA增强MR在动脉粥样硬化中的成像特点,比较两者对动脉粥样硬化斑块的检出率。方法:健康雄性新西兰大白兔35只,随机分为两组,实验组30只采用球囊导管损伤腹主动脉结合高脂饮食的方法建立兔动脉粥样硬化模型。对照组5只不做干预。12周后给予平扫、Gd-DTPA增强扫描、USPIO增强扫描。分别从两种对比剂增强的图像中各随机挑选97幅,与病理检查结果行对照研究,比较两者斑块的检出率。对照组任意取材对照。结果:实验组7只球囊损伤时意外死亡,2只死于喂养,1只死于实验麻醉,20只存活。对照组5只全部存活。制成172张病理切片,病理证实134个斑块组织,易损斑块72个。USPIO增强图像中经病理HE证实斑块84幅,检出率为86.6%;钆剂增强图像中HE切片证实斑块72幅,检出率为74.2%。斑块检出率比较χ2=3.96,P=0.046。结论:USPIO增强MRA对斑块的检出率明显高于传统钆剂增强MR对斑块的检出率。USPIO作为新型核磁对比剂对于动脉粥样硬化的检测具有明显的优越性。Objective To investigate the imaging characteristics of USPIO-enhanced and Gd-enhanced MRI in atherosclerosis and to compare the detection rate of atherosclerotic plaque between them. Methods Thirty-five healthy male rabbits were assigned to experimental group （n = 30） to establish a model of atherosclerosis by damaging aortic tunica intima with Foley＇s tube in combination with a high fat diet and 5 to control group without any intervention. At week 12, USPIO-enhanced and Gd-enhanced MRI scanning were conducted to compare the signal changes of atherosclerotic plaque before and after enhancement with the 2 contrast media. Ninety seven pictures were randomly selected respectively from the pictures enhanced by the 2 contrast media to compare the detection rate plaque between them. Pthology examination was used for detection standard. For the control group, pictures were randomly selected. Results In the experimental group, 7 rabbits died of Foley＇ s tube damaging, 2 died of raising and 1 died of anesthesia. All 5 rabbits in control group survived. A total of 172 pathological sections were made with 134 plaques and 72 vulnerable plaques pathologically confirmed. In pictures enhanced by USPIO, 84 plaques were confirmed by HE staining with a detection rate of 86.6%. In pictures enhanced by Gd, 72 plaques were confirmed by HE staining with a detection rate of 74.2%. Detection rate of USPIO-enhanced MRI in atherosclerosis plaque was significantly higher than that of Gd-enhanced MRI （X2 = 3.96, P = 0.046）. Conclusion USPIO shows its superiority as a new contrast medium in detection of atherosclerosis plaque.

关 键 词：动脉粥样硬化 MRI USPIO Gd—DTPA 

分 类 号：R541.4[医药卫生—心血管疾病]