京海黄鸡BMP-4基因突变位点的发现及其对生长和繁殖性状的遗传效应  

Detection of the Mutation of the BMP-4 Gene and Its Genetic Effect on Growth and Reproduction Traits in Jinghai Chicken

作　　者：张跟喜[1,2] 邢世宇 钮广林 胡东胜[1] 张向前[1] 王金玉[1,2] 

机构地区：[1]扬州大学动物科学与技术学院.江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009

出　　处：《中国畜牧杂志》2014年第5期6-9,共4页Chinese Journal of Animal Science

基　　金：扬州大学大学生科技创新基金(B12184);江苏省高校自然科学基金(12KJB230003);江苏高校优势学科建设工程项目;国家肉鸡产业技术体系(nycytx-42-G1-05)

摘　　要：本研究采用DNA池直接测序法寻找京海黄鸡BMP-4基因外显子4区域的多态位点,用PCR-SSCP技术检测发现的多态位点在京海黄鸡中的单核苷酸多态性,并分析其对京海黄鸡生长和繁殖性状的遗传效应。结果表明:在外显子4区域检测到2个突变位点(C701T,A967G);C701T位点经PCR-SSCP分析显示2种基因型(AA和AB),其中AA型为优势基因型。最小二乘分析表明,C701T位点对京海黄鸡的生长和繁殖性状均无显著影响(P>0.05)。The mutations in the exon 4 of the BMP-4 gene were detected in Jinghai Chicken using DNA pooling and sequencing. Single nucleotide polymorphisms （SNPs）of the found mutations were detected in Jinghai Chicken by PCR- SSCP. The genetic effects on growth and reproduction traits were also analysed. Results showed that two SNPs （C701T, A967G） were detected in the exon 4 of the BMP-4 gene. Two genetypes （AA and AB） were displayed by PCR-SSCP, and the genotype AA was the predominant genotype. The least square means analysis showed the differences for growth and reproductive traits among the 2 genotypes in Jinghai chicken were nonsignificant （P〉0.05）.

关 键 词：骨形态发生蛋白-4 多态性 遗传效应 京海黄鸡 

分 类 号：S831.2[农业科学—畜牧学]