天冬酰胺对脂多糖刺激仔猪肠黏膜结构及抗氧化能力的影响  

Effects of Asparagine on Intestinal Morphology and Antioxidant Capacity of Piglets Challenged with Lippolysaccharide

作　　者：皮定安[1] 刘玉兰[1] 李爽[1] 石海峰[1] 朱惠玲[1] 侯永清[1] 丁斌鹰[1] 冷炜博[1] 陈逢[1] 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023

出　　处：《中国畜牧杂志》2014年第5期60-64,共5页Chinese Journal of Animal Science

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0158);国家自然科学基金(31172222;31372318);湖北省自然科学基金项目(2010CDA050);湖北省国际科技合作项目(2011BFA008);武汉轻工大学研究生教育创新计划(2012cx005)

摘　　要：试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肠道结构及抗氧化能力的影响。选用24头断奶仔猪,分为对照组、LPS组、0.5%Asn组(0.5%Asn+LPS)和1.0%Asn组(1.0%Asn+LPS)。第24天,各组分别注射LPS或等量生理盐水24 h后屠宰。结果表明:LPS刺激导致空肠绒毛高度显著降低,日粮中添加0.5%或1.0%Asn显著降低了空肠、回肠的隐窝深度,提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度的比值(P<0.05);LPS刺激也导致空肠超氧化物歧化酶(SOD)活性和氧化型谷胱甘肽(GSSG)/还原性谷胱甘肽(GSH)比值显著升高(P<0.05);日粮中添加0.5%或1.0%Asn显著提高了空肠、回肠GSH的含量(P<0.05),降低了空肠、回肠GSSG/GSH的比值(P<0.01)和丙二醛(MDA)的含量(P<0.05)。结果显示,Asn缓解了LPS刺激引起的肠道结构损伤和抗氧化能力下降。This experiment was conducted to investigate the effects of asparagine （Asn） on the intestinal morphology andantioxidant capacity of piglets after lipopolysaccharide（LPS） challenge. Twenty-four piglets were allotted to four treatments： control group, LPS group, 0.5% Ash group （0.5% Asn＋LPS）, and 1.0% Asn group （1.0% Asn＋LPS）. On 24, the piglets were injected intraperitoneally with LPS or the same dose of physiological saline. At 24 h following LPS challenge, the pigs were slaughtered. The results showed that LPS pigs had a lower villus height in jejunum （P〈0.05）. Dietary supplementation of 0.5 or 1.0% Asn decreased crypt depth, and increased villus height and villus height/crypt depth ratio in jejunum and ileum （P〈0.05）. LPS pigs also had a higher activity of superoxide dismutase （SOD） and oxidized glutathione （GSSG）/reduced glutathione （GSH） ratio in jejunum （P〈0.05）. Dietary supplementation of 0.5 or 1.0% Asn had a higher GSH （P〈0.05）, and a lower GSSG/GSH ratio （P〈0.10） and malondialdehyde （MDA） level in jejunum and ileum （P〈0.05）. These results suggested that Asn alleviates the intestinal injury and oxidative stress induced by LPS challenge.

关 键 词：天冬酰胺 脂多糖 肠黏膜结构 抗氧化 仔猪 

分 类 号：S826.5[农业科学—畜牧学]