ω-3鱼油脂肪乳对慢性阻塞性肺疾病大鼠的干预作用  

Intervention of rat models of chronic obstructive pulmonery disease with ω-3 fatty acids

作　　者：殷宗宝[1] 向群[1] 计超[1] 谢斌[1] 侯宇[1] 

机构地区：[1]570208海口,海口市人民医院重症医学科

出　　处：《中华临床营养杂志》2014年第1期49-52,共4页Chinese Journal of Clinical Nutrition

摘　　要：目的 探讨ω-3鱼油脂肪乳对烟熏加气管注入脂多糖法致慢性阻塞性肺疾病（COPD）大鼠干预的作用机制.方法 清洁级雄性大鼠90只,完全随机分成生理盐水对照组、烟熏加气管注入脂多糖法致大鼠COPD模型组、烟熏加气管注入脂多糖法致大鼠COPD模型＋ω-3鱼油脂肪乳干预组.在第7、21、28天分别取左肺下叶供免疫组化检测核转录因子-κB （NF-κB）、干扰素-γ（IFN-γ）的表达,右肺下叶供HE染色病理观察；用ELISA双抗体夹心法测定各组大鼠血清白细胞介素-6（IL-6）、IFN-γ浓度.结果 （1）肺组织病理变化示第28天COPD模型组肺泡腔有大量炎性渗出,壁增厚,血管平滑肌增生,肺泡结构破坏；ω-3鱼油脂肪乳干预组肺组织结构部分破坏,肺泡腔少许炎性渗出；对照组大鼠几乎无肺泡炎发生.（2）第28天模型组大鼠肺组织NF-κB累计吸光度为18.91 ±3.07,显著高于对照组和干预组的5.47-±-4.86与7.23±2.21,差异均有统计学意义（均P＜0.01）；第28天模型组大鼠肺组织IFN-γ累计吸光度为7.12±3.37,显著低于干预组的18.74 ±2.65,差异有统计学意义（P＜0.01）.（3）第28天模型组大鼠血清IL-6为（13.43 ±2.47） ng/L,显著高于对照组和干预组的（4.78±1.93）和（4.98±1.89） ng/L,差异均有统计学意义（均P＜0.01）；第28天模型组大鼠血清IFN-γ为（2.23 ±0.63） ng/L,显著低于对照组和干预组的（4.51±0.71）和（7.05±0.52）ng/L,差异均有统计学意义（均P＜0.01）.结论 ω-3鱼油脂肪乳可以下调大鼠肺组织NF-κB,降低其血清含量和IL-6含量；上调肺组织1FN-γ表达,增加其血清含量,从两减轻COPD肺部炎症.Objective To explore the effectiveness of ω-3 fatty acids in intervening rat models of chronic obstructive pulmonery disease （COPD）.Methods The rat COPD models were established by cigarette smoking and intratracheal lipopolysaccharide instillation.Totally 90 rats were randomly divided into 3 groups：normal control group （treated with normal saline）,COPD group,and intervention group （the COPD rat models treated with ω-3 fatty acids）.Lung tissues were obtained on the 7th,21st,and 28th day.The left lower lobes were analyzed to determine the expressions of nuclear factor-kappa beta （NF-κB） and interferon-γ （IFN-γ）and the right lung lobes were sliced for detecting the cell apoptosis.Double antibody sandwich enzyme-linked immunosorbent assay （DAS-ELISA） was used to detect the serum IFN-γ and interleukin-6 （IL-6）.Results （1） The pathological changes of lung tissue：there were a large number of inflammatory exudation,alveolar wall thickening,hyperplasia of vascular smooth muscle and the alveolar structure destruction in the COPD model group,but the lung tissue were part of alveolar cavity and a little inflammatory exudate in ω-3 fatty fish acids treatment group,control rats were almost no alveolar inflammation on the 28th days.（2） On the 28th day,NF-κB protein expression of the lung tissue （18.91 ± 3.07） in rats of COPD model group was significantly higher than the control group and the intervention group （5.47 ±4.86 and 7.23 ±2.21） （P 〈0.01）.On the 28th day,IFN-γ protein expression in lung tissue of the rats in the model group was 7.12 ±3.37,significantly lower than the intervention group （18.74 ± 2.65）,the difference was statistically significant （P 〈 0.01）.（3） The IL-6 levels of the blood-serum of model group rats were （13.43 ± 2.47） ng/L,significantly higher than the control group and the intervention group [（4.78 ± 1.93） and （4.98 ± 1.89） ng/L],the differences were statistically significant （P 〈 0.01） on the 28th day

关 键 词：慢性阻塞性肺疾病 Ω-3鱼油脂肪乳 干扰素-Γ 核转录因子-ΚB 白细胞介素-6 

分 类 号：R563.9[医药卫生—呼吸系统]