新疆无苞芥锌指蛋白基因的克隆及表达分析  

Cloning and Expression Analysis of Zinc Finger Protein Gene from Olimarabidopsis pumila

作　　者：郭丹丽[1] 王崎崎 吴晓庆[1] 费春艳[1] 黄先忠[1] 

机构地区：[1]石河子大学生命科学学院,农业生物技术重点实验室,新疆石河子832003

出　　处：《西北植物学报》2014年第2期243-250,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31060149);新世纪优秀人才支持计划(NCET-12-1072);石河子大学高层次人才启动项目(RCZX200902);石河子大学SRP项目(SRP20130067)

摘　　要：利用同源克隆法从新疆无苞芥中克隆获得1个锌指蛋白基因(OpZFP)。序列分析表明,OpZFP基因的开放阅读框为684bp,推测编码含227个氨基酸的蛋白质。生物信息学分析显示,OpZFP蛋白含有1个典型的C2H2型锌指结构,在C端含有一个可能具有转录抑制功能的EAR结构域。系统进化树分析表明OpZFP编码产物与拟南芥AtZFP1、琴叶拟南芥AlZFP1的进化关系较近。分离了OpZFP基因2 095bp的启动子序列,发现该启动子与拟南芥AtZFP1基因的启动子序列只有84.4%的相似性,启动子分析表明二者存在多处不同的顺式作用元件。半定量RT-PCR分析表明,OpZFP在根、茎、叶、花和果荚中均有表达,在根中的表达量最高。OpZFP基因受高盐、干旱和低温等胁迫的诱导表达,表明该蛋白涉及多种胁迫相关的信号传导途径。Zinc finger protein plays important roles in plant stress tolerance. OpZFP, A zinc finger protein gene of Olirnarabidopsis pumila ,was isolated by homologous sequence cloning method. The open reading frame of OpZFP gene was 684 bp,encoding 227 amino acids which containing a typical Cys2/His2 zinc fin- ger domain,an EAR-motif at its C-terminal region. Phylogenetic analysis revealed that OpZFP had a closer relationship with AtZFP1 and A1ZFP1. A OpZFP promoter sequence covering 2 095 bp upstream of tran- scription start site was isolated from O. pumila,and showed only 84.4~ similarity with that of AtZFP1 gene promoter. Several different cis-elements were found analyzed in two promoters. Expression analysis by semi-quantitative RT-PCR indicated that OpZFP displayed a much broader expression range in different tissues in O. pumila,with a maximum expression in root. OpZFP gene was induced by high salt, drought and low temperature, suggesting that the function of OpZFP might involve in some stress related signal pathways.

关 键 词：无苞芥 锌指蛋白 ZFP基因 逆境 

分 类 号：Q785[生物学—分子生物学] Q786