DNA序列在悬钩子属植物分子系统学研究中的应用进展  

Research Progress of Molecular Phylogenetic Analyses Based on DNA Sequence Data in RubusL.(Rosaceae)

作　　者：张丽[1] 王小蓉[1,2] 王燕[1] 陈清[1] 何文[1] 

机构地区：[1]四川农业大学园艺学院,四川雅安625014 [2]四川农业大学果蔬研究所,成都611130

出　　处：《西北植物学报》2014年第2期423-430,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31272134)

摘　　要：悬钩子属植物种类繁多,类群复杂,而且多为多倍体和杂种。该文就近年来国内外有关DNA序列在悬钩子属植物分子系统学研究中的应用现状和进展进行了综述,并对中国悬钩子属植物系统发育研究进行了展望。研究认为:叶绿体DNA序列多应用非编码区,且多与ITS序列联合分析;核基因组中ITS序列应用最为广泛,主要用于研究悬钩子属空心莓组与木莓组的进化关系、栽培品种间亲缘关系及部分杂种和多倍体的起源等;在该属植物中发现了ITS个体内多态性,但未进行ITS假基因检测,其系统学应用价值需重新评价;低拷贝核基因只有GBSSI和LEAFY有相关应用。同时认为,悬钩子属植物系统学研究中应用的DNA序列及研究类群均较少,缺乏对整个悬钩子属全面而系统的研究。指出应进一步选择具有代表性的样本、筛选合适的DNA片段,并结合形态学、孢粉学和细胞学等手段对中国悬钩子属植物系统关系进行深入研究。Rubus is a large and taxonomically complex genus consisting of hybrid and polyploid. In this re- view, we summarized and discussed the application of DNA sequences in the Rubus L. phylogenetic studies, mainly focus on their limitation and promising application prospect in the Chinese Rubus. Noncoding chlo- roplast DNA fragments combined with ITS sequence were frequently applied. ITS was the most widely ap- plied sequence among the nDNAs. It has been used for clarifying the phylogeny between sections Idaeoba- tus and Malachobatus,illustrating genetic relationship among Rubus cultivars, and identifying the origin of hybrid and polyploid. Due to ITS polymorphism among individuals, it was necessary to detect putative pseudogenes. By contrast,low-copy nuclear genes were rarely applied except GBSSI and LEAFY. The phy- logenetic relationships of some Rubus species remain unclear because of the deficiency of available DNA se- quence and limitation of covered groups. Additionally, utilization of multi-specific DNA and more samples in phylogenetic applications probably has one bright future, along with the morphology, palynology, cytolo- gy and other means.

关 键 词：悬钩子属 DNA序列 系统发育 ITS序列 叶绿体DNA 低拷贝核基因 

分 类 号：Q789[生物学—分子生物学]