哺乳动物细胞表达系统研究进展  

Advances on Mammalian Cell Expression System

作　　者：李国坤[1] 高向东[1] 徐晨[2] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]北京三元基因工程有限公司,北京102600

出　　处：《中国生物工程杂志》2014年第1期95-100,共6页China Biotechnology

摘　　要：目前,哺乳动物细胞已成为生产多种生物药物的首选宿主细胞。哺乳动物细胞表达系统可进行翻译后修饰,表达的重组蛋白接近人源构象,因此被大量用于治疗性重组蛋白的生产,如何建立高效的哺乳动物细胞表达系统也受到研究者们的重视。随着基因组学、转录组学、蛋白质组学以及代谢组学研究不断深入,近几年来在优化哺乳动物表达系统方面取得了长足的进步。从高效表达载体构建、宿主细胞改造、高通量筛选、培养基优化等方面阐述哺乳动物细胞表达系统的研究进展,以期为研究者们提供一定的帮助。Mammalian cells have become the preferred host cells for the manufacture of a wide range of biopharmaeeuticals. Recombination proteins expressed by mammalian cell expression system approximate human forms due to post translation modification, and mammalian cell expression system is used widely to manufacture therapeutic recombinant proteins. The establishment of efficient expression system was paid close attention to by more researchers. Along with the development of genomics, transeriptomics, proteomics and metabolomics, great progress has been made in optimization of a mammalian expression system in recent years. Development of mammalian cell expression system through the construction of efficient expression vector, host cell transformation, high-throughput screening, and medium optimization were introduced.

关 键 词：高效表达载体 染色质开放元件 宿主细胞改造 高通量筛选培养基优化 

分 类 号：Q952[生物学—动物学]