2012年血培养双瓶阳性与单瓶阳性的比较及耐药性分析  

Comparison of two-bottle and one-bottle blood culture positive and drug resistance analysis in 2012

作　　者：姜森[1] 王辉[1] 李荷楠[1] 陈宏斌[1] 刘文云[1] 严薇[1] 

机构地区：[1]北京大学人民医院检验科,北京100044

出　　处：《中国卫生检验杂志》2014年第4期521-523,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的了解血培养双瓶与单瓶阳性在报阳时长与病原菌构成比的差异,在鉴别污染及临床诊断上的意义。方法对本院2012年送检的434份血培养阳性结果进行回顾性分析。结果在434份阳性标本中,双瓶阳性231份(53.23%),单瓶阳性185份(42.63%)。双瓶及多瓶血培养阳性中最常见细菌为大肠埃希菌(31.73%),而在单瓶阳性中最常见的是凝固酶阴性葡萄球菌(30.27%)。G-杆菌对碳青霉烯类抗生素耐药率最低;G+葡萄球菌对替加环素、替考拉宁、万古霉素及利奈唑烷的耐药率最低;肠球菌对替加环素及利奈唑烷的耐药率为最低。结论双瓶阳性在一定意义上有鉴别血培养污染的作用,坚持双份及以上血培养送检,降低污染率。Objective To explore the difference of two- bottle blood culture positive and one- bottle blood culture positive in positive alarm time and constituent ratio of pathogenic bacteria, as well as the significance in pollution differentiation and clinical diagnosis. Methods The results of 434 blood culture positive specimens were retrospectively analyzed in 2012. Results There were 231 two - bottle positive cases（53.23% ） and 185 one - bottle positive cases（42.63% ） in 434 positive samples. The most frequent pathogen was Escherichia coli in two - bottle and multi - bottle blood culture positive samples （31.73%）, while that was coagulase - negative staphylococcus in one - bottle blood culture positive samples （30.27 % ）. Drug resistance rate of G-bacillus was lowest to carbapenem antibiotics （imipenem, meropenem, ertapenem）, while that of G ＋ staphylococcus was lowest to tigecycline, teicoplanin, vancomycin, linezolid, that of enterococcus was lowest to tigecycline and linezolid. Conclu- sion Two - bottle blood culture positive was of significance in differentiation of blood cuhure contamination in a sense, it can reduce contamination rate.

关 键 词：血培养 报阳时长 病原菌 污染菌 耐药率 

分 类 号：R446.5[医药卫生—诊断学]